alexa De-Regulation of Extracellular Matrix Proteins in Human Fibroblasts after Long-term and Low Concentrations of HEMA Exposition
ISSN: 2157-7099

Journal of Cytology & Histology
Open Access

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Research Article

De-Regulation of Extracellular Matrix Proteins in Human Fibroblasts after Long-term and Low Concentrations of HEMA Exposition

Gabriella Teti1, Michela Zago2, Sandra Durante1, Stefano Focaroli1, Antonio Mazzotti3, Viviana Salvatore1, Matteo Cadossi3
and Mirella Falconi1*

1Department for Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, via Irnerio 48, Bologna, 40126 Italy

2Meakins-Christie Laboratories, McGill University, 3626 St. Urbain Street, Montreal, Quebec H2X 2P2, Canada

3Rizzoli Orthopedic Institute, Via di Barbiano 1/10, Bologna, 40136, Italy

*Corresponding Author:
Mirella Falconi
Department of Biomedical and Neuromotor Sciences (DIBINEM)
University of Bologna, via Irnerio, 48-40126 Bologna, Italy
Tel: +39 051 2091511
Fax: +39 051 251735
E-mail: [email protected]

Received date: February 26, 2014; Accepted date: March 25, 2014; Published date: March 27, 2014

Citation: Teti G, Zago M, Durante S, Focaroli S, Mazzotti A, et al. (2014) De-Regulation of Extracellular Matrix Proteins in Human Fibroblasts after Longterm and Low Concentrations of HEMA Exposition. J Cytol Histol 5:235. doi: 10.4172/2157-7099.1000235

Copyright: © 2014 Teti G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: 2 - hydroxyethyl methacrylate (HEMA) is one of the common components of most resin-based dental materials. Various studies have shown that HEMA can diffuse through dentin due to its low molecular weight and its hydrophilicity, and can affect the underlying odontoblast, cell division and activity. In this work, we have studied the influence of HEMA in regulating the expression of pro-collagen α1 type I and tenascin - C proteins in human fibroblasts after long term and low concentrations of HEMA.
Methods:
Human dental pulp cells were exposed to 0.1 mM and 0.5 mM for 1, 3, 5, 7, and 15 days. MTT assay, immunofluorescence and western blot analysis were carried out to investigate cell viability and modification in collagen type I and tenascin – C protein expression.
Results:
MTT assay showed an high cell viability, western blot and immunofluorescence demonstrated a down-regulation of collagen type I protein and un up-regulation of tenascin - C protein, the latter involved in cellular stress.
Conclusion:
low concentrations and long-term HEMA exposition, greatly influences the expression of collagen type I protein and tenascin – C protein in human dental pulp cells, modifying the extracellular matrix toward a stressful microenvironment.

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