Design and Manufacturing of Electromechanical Chip for Rapid Measurement of IgG1 Antibody in Cell-Culture SupernatantAzam Rahimpour1,2, Fatemeh Yazdian3, Zahra Akbari Jonoush4, Masoumeh Rajabibazl5 and Meisam Omidi2*
- *Corresponding Author:
- Omidi M
Medical Nano-Technology & Tissue Engineering Research Center
School of Advanced Technologies in Medicine
Shahid Beheshti University of Medical Sciences, Tehran, Iran
E-mail: [email protected]
Received Date: July 27, 2016; Accepted Date: August 26, 2016; Published Date: September 22, 2016
Citation: Rahimpour A, Yazdian F, Jonoush ZA, Rajabibazl M, Omidi M (2016) Design and Manufacturing of Electromechanical Chip for Rapid Measurement of IgG1 Antibody in Cell-Culture Supernatant. Biol Med (Aligarh) 8: 340. doi:10.4172/0974-8369.1000340
Copyright: © 2016 Omidi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this study, we have used arrays of piezoresistive nanomechanical membrane resonators (NMRs) to detect IgG1 antibody. The NMR consists of a membrane suspended by four piezoresistive sensing components. Unlike conventional cantilever mass sensors, our sensors retain a uniform mass sensitivity over the membrane surface. According to the experiments, it was revealed that NMRs have mass sensitivities in the order of 25 Hz/pg in air and 1.8 Hz/pg in liquid. This matter allows them to detect IgG1 over a wide range of concentrations from 100 pg/mL to 10 μg/mL in a background of human serum albumin (HSA) at 1 mg/ml. This indicates the fact that the self-sensing NMR approach is beneficial for detecting disease markers. Moreover, the performance of the NMR was compared with other detection methods, and the results indicated a better performance for NMR.