Detection and Identification of Avian Influenza Virus by cDNA Microarray
- *Corresponding Author:
- Calvin L Keeler Jr.
Department of Animal and Food Sciences
University of Delaware
Newark, DE 19716-2150, USA
Tel: 302 831-2524
E-mail: [email protected]
Received date: January 20, 2014; Accepted date: February 18, 2014; Published date: February 21, 2014
Citation: Jung Maughan MN, Bliss TW, Chung I, Suarez DL, Keeler CL Jr (2014) Detection and Identification of Avian Influenza Virus by cDNA Microarray. J Microb Biochem Technol S2:005. doi: 10.4172/1948-5948.S2-005
Copyright: © 2014 Maughan MN, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Influenza A viruses consisting of all known 16 HA and 9 NA subtypes have been isolated from birds. We have created a diagnostic avian cDNA microarray containing probes corresponding to the highly conserved matrix (M) gene, and selected hemagglutinin (HA), and neuraminidase (NA) subtypes of AIV. cDNA RT-PCR products from the HA, NA, and M genes of various avian influenza isolates and subtypes were used to create an avian influenza virus (AIV) cDNA microarray. The microarray was evaluated against a panel of AIV isolates in order to appraise its application in AIV detection and identification. Utilizing the M gene as a pan-influenza marker, all 10 samples were identified as being strains of type A influenza. The array was able to correctly HA- and NA-subtype subtype 7 out of 10 test samples. This included correctly identifying, subtyping, and determining the geographic origin of all of the H5 subtypes and the two H7 samples of U.S. origin.