alexa Detection and Quantification of Protein Post-Translational Modifications Using Novel Microchannel Plate Autoradiographic Imagers
ISSN: 2161-0444

Medicinal Chemistry
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Detection and Quantification of Protein Post-Translational Modifications Using Novel Microchannel Plate Autoradiographic Imagers

Wayne Grant Carter*

School of Graduate Entry Medicine & Health, University of Nottingham Medical School, Royal Derby Hospital Centre, Derby DE22 3DT, UK

*Corresponding Author:
Wayne Grant Carter
School of Graduate Entry Medicine & Health,
University of Nottingham Medical School
Royal Derby Hospital Centre Derby DE22 3DT, UK,
Tel: +44 (0)1332 724738
Fax: +44 (0)1332 724626
E-mail: [email protected]

Received date: November 17, 2012; Accepted date: November 23, 2012; Published November 26, 2012

Citation: Carter WG (2012) Detection and Quantification of Protein Post-Translational Modifications Using Novel Microchannel Plate Autoradiographic Imagers. Med chem S11:001. doi:10.4172/2161-0444.S11-001

Copyright: © 2012 Carter WG. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

It is likely that all proteins are modified post-translationally, either enzymatically or non-enzymatically. Scientists have continued to develop suitable methodologies in order to study the ever burgeoning list of protein post-translational modifications (PTMs). For the study of PTMs, one must consider suitable assays by which the target protein can be detected, the site(s) of PTM determined, the stoichiometry and stability of the PTM evaluated, and the biological outcome of the modification assessed. Historically, one of the methods of choice for identifying which protein is modified, and the site and stoichiometry of modification, has been the utilisation of commercial radiochemicals. Of these, tritium (3H) has excellent potential to facilitate analyses of biochemical reactions, but has the weakest signal strength of the commonly employed biological β-emitters. Hence development of imaging devices that are superior to conventional film autoradiography will enable a better exploitation of the universal application of 3H for measuring PTMs. Herein, we discuss the current application of film autoradiography and the advantages of using microchannel plate (MCP) autoradiographic imagers.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords