Detection of BRAF V600E Mutation in Langerhans Cell Histiocytosis Using High-resolution Melting Analysis in Decalcified, Paraffin-embedded TissueBin Fu1,2 Zhongqing Wang2, Xiaolin Li2, Sa A Wang3 and Zhuang Zuo3*
- *Corresponding Author:
- Zhuang Zuo, MD, PhD
Department of Hematopathology
Unit 149, The University of Texas MD Anderson Cancer Center
1515 Holcombe Blvd, Houston, TX 77030, USA
E-mail: [email protected]
Received date Januray 29, 2013; Accepted date March 20, 2013; Published date March 22, 2013
Citation: Fu B, Wang Z, Li X, Wang SA, Zuo Z (2013) Detection of BRAF V600E Mutation in Langerhans Cell Histiocytosis Using High-resolution Melting Analysis in Decalcified, Paraffin-embedded Tissue. J Leuk (Los Angel) 1:101. doi: 10.4172/2329-6917.1000101
Copyright: © 2013 Fu B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The oncogenic BRAF V600E mutation has recently been found in a substantial number of Langerhans cell histiocytosis (LCH) cases. We developed and validated a rapid and sensitive high-resolution melting (HRM) analysis–based assay to detect BRAF V600E mutation in formalin-fixed, paraffin-embedded (FFPE), decalcified bone tissues, including 32 LCH cases and four primary bone lymphoma cases. BRAF V600E mutation was found in 18 (56%) of the LCH cases. HRM results were confirmed by Sanger sequencing. Our assay is thus a rapid, sensitive, and low-cost way to detect the BRAF V600E mutation in LCH FFPE samples for clinical diagnostic use.