Detection of c-MYC Gene in Micronucleated Hepatocytes from Regenerative Cirrhotic Nodules and Hepatocellular Carcinoma of Hepatitis C Virus Infected PatientsTerezinha Morato Bastos de Almeida1, Regina Maria Cubero Leitão2, Maisa Yoshimoto3, Joyce Anderson Duffles Andrade3, Willy Beçak4, Flair José Carrilho5 and Shigueko Sonohara1*
- *Corresponding Author:
- Shigueko Sonohara
Department of Oncology, Radiology and Oncology
Faculty of Medicine, University of Sao Paulo
Sao Paulo, Brazil
E-mail: [email protected]
Received date: June 28, 2012; Accepted date: August 20, 2012; Published date: August 22, 2012
Citation: de Almeida TMB, Cubero Leitão RM, Yoshimoto M, Andrade JAD, Beçak W, et al. (2012) Detection of c-MYC Gene in Micronucleated Hepatocytes from Regenerative Cirrhotic Nodules and Hepatocellular Carcinoma of Hepatitis C Virus Infected Patients. J Carcinogene Mutagene 3:130. doi: 10.4172/2157-2518.1000130
Copyright: © 2012 de Almeida TMB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
c-MYC gene alteration has already been shown in chronic liver diseases, which includes hepatocellular carcinoma (HCC). The spontaneous formation of micronucleated hepatocytes (MN-Heps) in liver cirrhosis (LC) tissue from patients infected by hepatitis C virus (HCV), has been previously reported. The objective of this study was to investigate if the c-MYC gene sequence is lost by MN-Heps in cirrhotic process with or without HCC. For this purpose the presence of c-MYC gene in MN-Heps was investigated by fluorescence in situ hybridization in paraffin-embedded liver tissue. Five control liver samples of healthy organ donors were included in this study. Increased c-MYC gene copies were detected in 48-66% of MN-Heps in cirrhotic nodules from all cases, but not in control liver cells. Furthermore, gain of c-MYC gene number of copies was detected in 28% of the hepatocytes from regenerative and macroregenerative nodules (RNs, MRNs). The increase was also determined in 46% of tumor cells. No significant difference in extrusion of the c-MYC gene by RN or MRN hepatocytes was observed. We concluded that RNs and MRNs show cytogenetic abnormality towards the c-MYC gene. Its extrusion in MN-Heps seems to be an early event in regenerative cirrhotic lesions. The increase of c-MYC gene copies in LC from HCV infected patients might contribute to the development of HCC.