alexa Detection of c-MYC Gene in Micronucleated Hepatocytes from Regenerative Cirrhotic Nodules and Hepatocellular Carcinoma of Hepatitis C Virus Infected Patients
ISSN: 2157-2518

Journal of Carcinogenesis & Mutagenesis
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Research Article

Detection of c-MYC Gene in Micronucleated Hepatocytes from Regenerative Cirrhotic Nodules and Hepatocellular Carcinoma of Hepatitis C Virus Infected Patients

Terezinha Morato Bastos de Almeida1, Regina Maria Cubero Leitão2, Maisa Yoshimoto3, Joyce Anderson Duffles Andrade3, Willy Beçak4, Flair José Carrilho5 and Shigueko Sonohara1*

1Department of Oncology, Radiology and Oncology, Faculty of Medicine, University of Sao Paulo, Sao Paulo, Brazil

2Laboratory of Pathology Diagnóstika, Sao Paulo, Brazil

3Institute of Pediatric Oncology, University of Sao Paulo, Sao Paulo, Brazil

4Laboratory of Genetics, Instituto Butantan, São Paulo, Brazil

5Department of Clinical Gastroenterology-Hepatology, Faculty of Medicine, University of Sao Paulo, Sao Paulo, Brazil

*Corresponding Author:
Shigueko Sonohara
Department of Oncology, Radiology and Oncology
Faculty of Medicine, University of Sao Paulo
Sao Paulo, Brazil
Tel: 055-11-30617470
Fax: 055-11-30826580
E-mail: [email protected]

Received date: June 28, 2012; Accepted date: August 20, 2012; Published date: August 22, 2012

Citation: de Almeida TMB, Cubero Leitão RM, Yoshimoto M, Andrade JAD, Beçak W, et al. (2012) Detection of c-MYC Gene in Micronucleated Hepatocytes from Regenerative Cirrhotic Nodules and Hepatocellular Carcinoma of Hepatitis C Virus Infected Patients. J Carcinogene Mutagene 3:130. doi: 10.4172/2157-2518.1000130

Copyright: © 2012 de Almeida TMB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

c-MYC gene alteration has already been shown in chronic liver diseases, which includes hepatocellular carcinoma (HCC). The spontaneous formation of micronucleated hepatocytes (MN-Heps) in liver cirrhosis (LC) tissue from patients infected by hepatitis C virus (HCV), has been previously reported. The objective of this study was to investigate if the c-MYC gene sequence is lost by MN-Heps in cirrhotic process with or without HCC. For this purpose the presence of c-MYC gene in MN-Heps was investigated by fluorescence in situ hybridization in paraffin-embedded liver tissue. Five control liver samples of healthy organ donors were included in this study. Increased c-MYC gene copies were detected in 48-66% of MN-Heps in cirrhotic nodules from all cases, but not in control liver cells. Furthermore, gain of c-MYC gene number of copies was detected in 28% of the hepatocytes from regenerative and macroregenerative nodules (RNs, MRNs). The increase was also determined in 46% of tumor cells. No significant difference in extrusion of the c-MYC gene by RN or MRN hepatocytes was observed. We concluded that RNs and MRNs show cytogenetic abnormality towards the c-MYC gene. Its extrusion in MN-Heps seems to be an early event in regenerative cirrhotic lesions. The increase of c-MYC gene copies in LC from HCV infected patients might contribute to the development of HCC.

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