Detection of DNA Damage Response Caused by Different Forms of Titanium Dioxide Nanoparticles using Sensor Cells
- *Corresponding Author:
- Akiyoshi Taniguchi
Director of Cell-Materials Interaction Group
National Institute for Materials Science (NIMS)
1-1, Namiki, Tsukuba, Ibaraki
E-mail: [email protected]
Received Date: October 01, 2012; Accepted Date: October 26, 2012; Published Date: October 28, 2012
Citation: Chen P, Taniguchi A (2012) Detection of DNA Damage Response Caused by Different Forms of Titanium Dioxide Nanoparticles using Sensor Cells. J Biosens Bioelectron 3:129. doi: 10.4172/2155-6210.1000129
Copyright: © 2012 Chen P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Titanium dioxide nanoparticles (TiO2 NPs) are generally considered to be biologically inert. However, TiO2 occurs in several crystalline forms, the two most common being rutile and anatase. Although both forms are tetragonal, the different crystalline forms give rise to different physical and chemical characteristics such as hardness, refractive index and photocatalytic ability. We hypothesized that the two forms of TiO2 NPs would also elicit different cellular responses. Three cell-based biosensors, using B-cell Translocation Gene 2 (BTG2), heat shock protein70B' (HSP70B') and nuclear factor kappa B (NF-ÃÂºB) sensor cells, were used to determine if the different forms of TiO2 NPs cause different cellular responses. The cellular responses induced by TiO2 NPs were detected using HSP70B' and NF-ÃÂºB sensor cells; we found that the different forms of TiO2 NPs resulted in the same HSP70B' and NF-ÃÂºB response. BTG2 expression is up-regulated by DNA damage via p53 activation. A cellular DNA damage response stimulated by different forms of TiO2 NPs was detected by our cell-based DNA damage biosensor. The results showed that an increased DNA damage response is elicited by the anatase form compared to the rutile or mixed rutile/anatase forms. Our work indicates that the crystalline form of NPs is an important point to investigate when studying the interaction between nanomaterials and cells.