alexa Detection of Extended Spectrum Beta-Lactamase Producing
ISSN: 2375-4397

Journal of Pollution Effects & Control
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Research Article

Detection of Extended Spectrum Beta-Lactamase Producing Escherichia Coli on Water at Hafr Al Batin, Saudi Arabia

Sulaiman Ali Al Yousef, Eman Saleh Farrag, Ahmed Mohamed Ali, Sabry Younis Mahmoud*
Department of Medical Laboratory Technology, College of Applied Medical Science, University of Hafr Al Batin, Kingdom of Saudi Arabia
*Corresponding Author : Sabry Younis Mahmoud
Department of Medical Laboratory Technology
College of Applied Medical Science
University of Hafr Al Batin
Kingdom of Saudi Arabia
Tel: +966506892734
E-mail: [email protected]
Received January 25, 2016; Accepted February 24, 2016; Published February 29, 2016
Citation: Al Yousef SA, Farrag ES, Ali AM, Mahmoud SY (2016) Detection of Extended Spectrum Beta-Lactamase Producing Escherichia Coli on Water at Hafr Al Batin, Saudi Arabia. J Pollut Eff Cont 4:155. doi:10.4172/2375-4397.1000155
Copyright: © 2016 Al Yousef SA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


In this study antibiotic resistant Escherichia coli from household water collected in Hafr AlBatin, Saudi Arabia, were characterized by prevalence of extended spectrum betalactamase (ESBL). Samples were collected from drinking and washing water at 12 locations. From the 144 samples obtained, Millipore membrane filters incubated on trypton soya agar plates produced colonies that yielded 34 isolates of E. coli as verified by biochemical tests. Isolates suspected ESBL producing were tested by using MicroScan analysis and disc diffusion test as ESBL phenotypic confirmatory methods. Phenotypically confirmed ESBL isolates were examined for antimicrobial susceptibility against 30 antibiotics and amplification of blaVEM, blaCTX, blaTEM, blaGES and blaSHV genes by polymerase chain reaction. Out of 34 E. coli isolates, only 6 (17.6%) were positive for ESBL producing according to MicroScan analysis. Disk diffusion as a confirmatory test indicates sensitivity of MicroScan system. PCR results indicated that the VEB was the most prevalent (83.3%) followed by CTX gene (16.6%) between these isolates. The ESBL-producing E. coli isolates was fully susceptible to pip/tazo (100%) and fully resistance to ampicillin, cefazolin and pepracillin (100%). This study showed that ESBL-producing E. coli are multidrug-resistant and existent in Hafr Al Batin’s water. Also, data indicated that wastewater maybe contributes as a source and reservoir of antibiotic resistance.


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