Detection of Group B Streptococcus agalactiae from Anorectal and Vaginal Screening Tests
Marcos André Schörner, Otto Henrique May Feuershuette, Mara Cristina Scheffer, Simone Gonçalves Senna, Maria Luiza Bazzo and Rosemeri Maurici*
Federal University of Santa Catarina, Florianópolis, Santa Catarina Brazil
- *Corresponding Author:
- Rosemeri Maurici
Professor, Internal Medicine
Federal University of Santa Catarina
Rodovia Virgílio Várzea, 2236, 601-A, Saco Grande
Florianópolis, Santa Catarina 88032001, Brazil
E-mail: [email protected]
Received date: July 26, 2014; Accepted date: September 29, 2014; Published date: October 08, 2014
Citation: Schörner MA, May Feuershuette OH, Scheffer MC, Senna SG, Bazzo ML (2014) Detection of Group B Streptococcus agalactiae from Anorectal and Vaginal Screening Tests. Clin Microbial 3:169. doi: 10.4172/2327-5073.1000169
Copyright: © 2014 Schörner MA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: The aim of the present study was to analyze two DNA extraction methods for use in molecular GBS diagnostics and compare them to the results of culture method.
Materials and methods: Two hundred vaginal samples were collected during the antenatal period, as per CDC recommendations, and atr gene polymerase chain reaction (PCR) was performed.
Results: Comparison of the two DNA extraction methods demonstrated 45% concordance. Sensitivity and specificity for 5 M Guanidine DNA extraction were 100% and 86.5%, respectively. Sensitivity and specificity for the commercial DNA extraction kit were 50% and 95%, respectively.
Conclusion: This study demonstrated that 5 M Guanidine DNA extraction was superior to the commercial kit, with PCR presenting a shorter turnaround time than culture. PCR could improve sensitivity and, therefore, may be a useful screening method. Sensitive GBS diagnosis allows for an effective treatment, with decreased newborn morbidity and mortality; therefore, cost-effectiveness studies are necessary to assess the feasibility of implementing PCR in routine laboratories, together with maternity ward collaboration.