alexa Detection of Growth Hormone Releasing Peptides in Serum by a Competitive Receptor Binding Assay | OMICS International | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

Detection of Growth Hormone Releasing Peptides in Serum by a Competitive Receptor Binding Assay

Ferro P1*, Krotov G2, Zvereva I2, Pérez-Mana3,4, Mateus JA3 and Segura J1,5

1Bioanalysis Research Group, Neuroscience Research Program, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain

2Anti-Doping Centre, Moscow, Russia

3Integrative Pharmacology and Systems Neuroscience Research Group, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain

4Department of Pharmacology, Therapeutics and Toxicology, Autonomous University of Barcelona, Cerdanyola, Spain

55Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), Barcelona Biomedical Research Park, Barcelona, Spain

*Corresponding Author:
Ferro P
Bioanalysis Research Group
Neuroscience Research Program
IMIM (Hospital del Mar Medical Research Institute)
Barcelona, Spain
Tel: +34 933160470
E-mail: [email protected]

Received date: February 02, 2017; Accepted date: February 15, 2017; Published date: February 21, 2017

Citation: Ferro P, Krotov G, Zvereva I, Pérez-Mana, Mateus JA, et al. (2017)Detection of Growth Hormone Releasing Peptides in Serum by a Competitive Receptor Binding Assay. J Chromatogr Sep Tech 8:351. doi: 10.4172/2157-7064.1000351

Copyright: © 2017 Ferro P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The use of small peptides for medical purposes has gained great importance in the last decade. Among these molecules are the growth hormone releasing peptides (GHRPs). These substances are highly sought by cheating athletes because of their ergogenic effects. Thus, GHRPs are listed in the World Antidoping Agency Prohibited List and several methods have been developed for their detection in urine samples. Serum is another useful matrix frequently used for anti-doping control. An experimental adaptation of a competitive methodology for GHRPs wide-group detection, previously developed for urine, is reported here using a serum matrix instead. The modifications have been mainly addressed the need to remove endogenous ghrelin (always present in serum) to avoid interference in the competitive binding assay. The modified test was first evaluated using serum spiked with different pure GHRPs (i.e., GHRP-2, GHRP-6 and Hexarelin), then using serum samples from pilot studies in which healthy subjects were treated intravenously with GHRP-2 and intranasally with GHRP-2 and GHRP-6. The results showed the reliability of the modified methodology for the detection of GHRPs in a serum matrix.

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