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Detection of OXA-51 Carbapenemase Gene in Klebsiella pneumoniae: A Case Report and a New Dimension on Carbapenemase Resistance | OMICS International | Abstract
ISSN: 1747-0862

Journal of Molecular and Genetic Medicine
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Research Article

Detection of OXA-51 Carbapenemase Gene in Klebsiella pneumoniae: A Case Report and a New Dimension on Carbapenemase Resistance

Budak S1, Aktaş Z2*, Oncul O1, Acar A1, Ozyurt M3, Turhan V1 and Gorenek L1

1Department of Infectious Disease and Clinical Microbiology, Gulhane Military Medical Academy Haydarpasa Training Hospital, Uskudar-Istanbul 34668, Turkey

2Department of Clinical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey

3Department of Clinical Microbiology, Gulhane Military Medical Academy Haydarpasa Training Hospital, Uskudar-Istanbul, Turkey

Corresponding Author:
Zerrin Aktas
Department of Clinical Microbiology
Istanbul Faculty of Medicine
Istanbul University,Capa-34390, Istanbul, Turkey
Tel:
0090212 414 20 00-32279
Fax: 0090212 414 20 37
E-mail: [email protected]

Received date: July 09, 2013; Accepted date: August 06, 2013; Published date: August 08, 2013

Citation: Budak S, Aktas Z, Oncul O, Acar A, Ozyurt M, et al. (2013) Detection of OXA-51 Carbapenemase Gene in Klebsiella pneumoniae: A Case Report and a New Dimension on Carbapenemase Resistance. J Mol Genet Med 7:63. doi:10.4172/1747-0862.1000063

Abstract

We investigated the occurence of genes associated with the production of carbapenem hydrolysing carbapenemases in Klebsiella pneumoniae isolates recovered from ICU patient. Antimicrobial susceptibility testing was performed and resistance genes were characterized by PCR amplification and sequencing. The Modified Hodge Test (MHT), MBL E-test, EDTA and aminophenylboronic acid (APBA) combined disk diffusion method, and a disk enzymatic assay were performed for the screening of carbapenemases. MICs of carbapenems were as follows (mg/L) ertapenem 8, meropenem 1, imipenem 0.25 and doripenem 0.5. The isolate demonstrated positive results in the ESBL, EDTA and APBA combined tests, and disk enzymatic assay. PCR and sequencing revealed the presence of blaOXA-51and blaCTX-M-15 beta-lactamase genes. Plasmids were not transferred to recipient E.coli by conjugation and transformation. In conclusion, we report on the first detection of the OXA-51 harboring K.pneumoniae isolate and co-produced a CTX-M-15 β-lactamase.

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