alexa Detection of tdh and trh Toxic Genes in Vibrio Alginolyticus Strain from Mantis Shrimp (Oratosquilla Oratoria)
ISSN: 2155-9600

Journal of Nutrition & Food Sciences
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Research Article

Detection of tdh and trh Toxic Genes in Vibrio Alginolyticus Strain from Mantis Shrimp (Oratosquilla Oratoria)

Gargouti AS1, Ab-Rashid MNK1, Ghazali MF1, Mitsuaki N2, Haresh KK3 and Radu S1*

1Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

2Centre for South Asia Studies, Kyoto University, Kyoto 606-8510, Japan

3Department of Microbiology, Faculty of Medicine, Mahsa University, 59100, Kuala Lumpur, Malaysia

*Corresponding Author:
Radu S
Department of Food Science
Faculty of Food Science and Technology
Universiti Putra Malaysia, 43400 UPM
Serdang, Selangor Darul Ehsan, Malaysia
Tel: +60389468361
Fax: +60389423552
E-mail: [email protected]

Received Date: August 07, 2015; Accepted Date: August 18, 2015; Published Date: August 21, 2015

Citation: Gargouti AS, Ab-Rashid MNK, Ghazali MF, Mitsuaki N, Haresh KK, etal. (2015) Detection of tdh and trh Toxic Genes in Vibrio alginolyticus Strain from Mantis Shrimp (Oratosquilla Oratoria). J Nutr Food Sci 5:405. doi: 10.4172/2155- 9600.1000405

Copyright: © 2015 Gargouti AS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Vibrio alginolyticus is a halophilic Gram-negative marine bacterium. The current study investigated the presence of the tdh and trh virulence factors in isolates of Vibrio alginolyticus. Five samples of Mantis Shrimp (Oratosquilla oratoria) were collected from local markets around Selangor, Malaysia. Samples were then examined using the MPN and multiplex PCR methods. By targeting the species-specific gene toxR, 2 (40%) were found to be positive. The two samples were positive for Vibrio alginolyticus, and one of these samples contained tdh/trh toxin. The Vibrio alginolyticus isolated, possessed and expressed the trh, tdh and toxR toxic genes. Vibrio strain identification was determined by 16S rRNA sequence; the results showed 99% homology to Vibrio alginolyticus. The sequencing of the isolated toxin genes indicated a homology of 97-98% to the Vibrio parahaemolyticus toxin genes. To our knowledge, this is the first report of Vibrio alginolyticus possessing three toxin genes of Vibrio parahaemolyticus.

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