alexa Detection of the Presence of Bordetella pertussis by Real-Time Polymerase Chain Reaction in Children Diagnosed with Pertussis and among their Household Contacts | Abstract
ISSN: 2157-7560

Journal of Vaccines & Vaccination
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Research Article

Detection of the Presence of Bordetella pertussis by Real-Time Polymerase Chain Reaction in Children Diagnosed with Pertussis and among their Household Contacts

Zehra Diyar Tamburaci Uslu1*, Mehmet Ceyhan2, Ener Cagri Dinleyici3, Zafer Kurugol4, Bedriye Nuray Alpman1, Eda Karadag Oncel2, Venhar Gurbuz2, Emre Aycan2, Hasan Tezer5 and Belgin Gulhan6

1Clinic of Pediatrics, Cankiri State Hospital, Cankiri, Turkey

2Department of Pediatrics, Unit of Infectious Diseases, Hacettepe University School of Medicine, Ankara, Turkey

3Department of Pediatrics, Osmangazi University School of Medicine, Eskisehir, Turkey

4Department of Pediatrics, Ege University School of Medicine, Izmir, Turkey

5Department of Pediatrics, Unit of Infectious Diseases, Gazi University School of Medicine, Ankara, Turkey

6Clinic of Pediatrics, Diskapi Training and Research Hospital, Ankara, Turkey

*Corresponding Author:
Zehra Diyar Tamburaci Uslu
Dumlupinar Bulvari
Akdeniz Universitesi Hastanesi 07059
Kampus, Antalya, Turkey
Tel: 00905057664383
Fax: 00902422274320
E-mail: [email protected]

Received date: April 17, 2013; Accepted date: August 12, 2013; Published date: August 17, 2013

Citation: Tamburaci Uslu ZD, Ceyhan M, Dinleyici EC, Kurugol Z, Alpman BN, et al. (2013) Detection of the Presence of Bordetella pertussis by Real-Time Polymerase Chain Reaction in Children Diagnosed with Pertussis and among their Household Contacts. J Vaccines Vaccin 4:199. doi: 10.4172/2157-7560.1000199

Copyright: © 2013 Tamburaci Uslu ZD, et al. This is an open-access articledistributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Background: Despite high vaccination coverage, pertussis continues to be a great public health problem. Adults and adolescents are source of infection for infants who are too young to be completely vaccinated. The aim of the study is detect if there is a household transmission of pertussis. Methods: Presence of Bordetella pertussis among the household contacts of the children who had had the positive result for Bordetella pertussis by real-time polymerase chain reaction was detected. Results: Of the 173 infants with clinical suspicion of pertussis, 48 (27.7%) turned out to be positive by PCR. The mothers of 19 (41.3% of all tested mothers) of them were also PCR positive for B. pertussis, being the family member most frequently infected. Conclusion: Despite high coverage of pertussis vaccination in Turkey, pertussis is prevalent both in incompletely protected infants and older children. High rate of presence of the bacteria in the household contacts depicts the importance of further pertussis booster vaccination in adolescents, adults and the targeted vaccination of the adults having close contacts with infants (cocoon strategy).

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