alexa Determination and Isolation of Ginsenosides from Aralia
ISSN: 2329-6836

Natural Products Chemistry & Research
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Research Article

Determination and Isolation of Ginsenosides from Araliaceous Plants by using Eastern Blotting Fingerprinting

Nguyen Huu Tung1, Noriko Fukuda2, Hiroyuki Tanaka2 and Yukihiro Shoyama1*
1Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan
2Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka 812-8582, Japan
Corresponding Author : Yukihiro Shoyama
Professor, Faculty of Pharmaceutical Sciences, Nagasaki
International University, Nagasaki 859-3298, Japan
Tel: +81- 956-20-5653
Fax: +81-956-20-5653
E-mail: [email protected]
Received March 28, 2013; Accepted April 24, 2013; Published April 30, 2013
Citation: Tung NH, Fukuda N, Tanaka H, Shoyama Y (2013) Determination and Isolation of Ginsenosides from Araliaceous Plants by Using Eastern Blotting Fingerprinting. Nat Prod Chem Res 1:107. doi: 10.4172/2329-6836.1000107
Copyright: © 2013 Tung NH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

In the eastern blotting method, a new fashion to separate the ginsenoside molecule into two functional parts using a simple and well-known chemical reaction was developed. In principle, the sugar moieties were oxidized by NaIO4 to form dialdehydes, which reacted with amino groups of the protein and covalently bound to the adsorbent PVDF membrane. The MAb bound to the aglycon part of the ginsenoside molecule for immunostaining. Double staining of eastern blotting for ginsenosides using anti-ginsenoside -Rb1 and –Rg1 MAbs promoted complete identification of ginsenosides in Panax species. As an application, we analyzed several Araliaceous plants by eastern blotting and enzyme-linked immunosorbent assay (ELISA) using anti-ginsenoside Rb1 MAb leading to the investigation of ginsenoside Rb1 from Kalopanax pictus and Acanthopanax koreanum. On the other hand, by immunoassay-guided fractionation and chromatography separation on the methanol extract of American ginseng, two new minor ginsenosides were isolated. As another application, identification of two known ginsenosides was achieved from the P. japonicas extract using eastern blotting and immunoaffinity column combined with anti-ginsenoside Rb1 MAb.

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