Determination of Fragrance Allergens in Essential Oils and Evaluation of their in vitro Permeation from Essential Oil Formulations through Cultured Skin
Lai-Hao Wang* and Hung-Jiun Liu
Department of Applied Chemistry, Chia Nan University of Pharmacy and Science, Tainan 71743, Taiwan
- *Corresponding Author:
- Dr. Lai-Hao Wang,
Department of Applied Chemistry
Chia Nan University of Pharmacy and Science
Tainan 71743, Taiwan
E-mail: [email protected]inet.net
Received Date: May 09, 2010; Accepted June 10, 2010; Published Date: June 10, 2010
Citation:Wang LH, Liu HJ (2010) Determination of Fragrance Allergens in Essential Oils and Evaluation of their in vitro Permeation from Essential Oil Formulations through Cultured Skin. J Bioequiv Availab 2: 072-076. doi: 10.4172/jbb.1000034
Copyright: © 2010 Wang LH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
We developed a high-performance liquid chromatography method using fl uorometric detection to simultaneously determine the allergens in fragrances, primarily structurally related allylbenzenes in essential oils. The effect of essential oil carriers—cloves, basil, and cinnamon—on the release and percutaneous absorption of the oils was studied in vitro using a cultured epidermal autograft membrane model.