alexa Determination of Genetic Similarities in Natural Popula
ISSN: 2168-9547

Molecular Biology: Open Access
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Determination of Genetic Similarities in Natural Populations of Drosophila melanogaster in Savanna Zone of Nigeria Using Microsatellite Markers

Abdulazeez R1*, Auta J1, Shehu DM1, Ndams IS1and Umar AU2

1Department of Biological Sciences, Ahmadu Bello University, Zaria, Nigeria

2National Animal Production Research Institute, Ahmadu Bello University, Shika, Zaria, Nigeria

*Corresponding Author:
Abdulazeez R
Department of Biological Sciences
Ahmadu Bello University, Zaria, Nigeria
Tel: +234(0)7033701917
E-mail: [email protected]

Received date: October 07, 2016; Accepted date: October 17, 2016; Published date: October 24, 2016

Citation: Abdulazeez R, Auta J, Shehu DM, Ndams IS, Umar AU (2016) Determination of Genetic Similarities in Natural Populations of Drosophila melanogaster in Savanna Zone of Nigeria Using Microsatellite Markers. Mol Biol 5:177. doi:10.4172/2168-9547.1000177

Copyright: © 2016 Abdulazeez R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

This study was carried out to determine genetic similarities in natural population of Drosophila melanogaster in savannah zone of Nigeria using microsatellite markers. A total of 184 D. melanogaster was collected using simple traps baited with banana and baker’s yeast from Northern Guinea (Kaduna), Sudan (Kano) and Sahel (Azare). A total of 40 D. melanogaster were genotyped using 7 sets of microsatellite markers, the bands in the electrophoregramme were analysed and scored using the image LabTM. The result was used to compute dendrogramme based on Nei’s genetic distance using MEGA 6. Result showed that studied population forms two (2) clusters at various degrees of similarities with Sudan savanna and Sahel Savanna forming a cluster at 0.21 then grouped with Northern Guinea Savanna to form the second (2nd) cluster at 0.78 distances, suggesting that Northern Guinea Savanna and Sudan Savanna Drosophila melanogaster populations have a recent common ancestor.

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