Determination of Metoclopramide Hydrochloride in Pharmaceutical Formulations Using Three Different Spectrophotometric MethodsNaggar AH*, Elnasr TAS, Sayed Ali AS, Kotb A and El Sayed AAY
Chemistry Department, Faculty of Science, Al–Azhar University, 71524, Assiut, Egypt.
- *Corresponding Author:
- Naggar AH
Chemistry Department, Faculty of Science
Al–Azhar University, Assiut Branch, 71524
Email: [email protected]
Received date: October 20, 2016; Accepted date: February 25, 2017; Published date: February 28, 2017
Citation: Naggar AH, Elnasr TAS, Sayed Ali AS, Kotb A, El Sayed AAY (2017) Determination of Metoclopramide Hydrochloride in Pharmaceutical Formulations Using Three Different Spectrophotometric Methods. Pharm Anal Acta 8:538. doi: 10.4172/2153-2435.1000538
Copyright: © 2017 Naggar AH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Three simple, selective, accurate and precise spectrophotometric methods were developed for the determination of Metoclopramide Hydrochloride (MCP) in dosage forms. The first method (Method A) is based on coupling reaction of diazotized MCP with 2,5-diphenyl-2,4-dihydro-pyrazol-3-one (DPP) in alkaline medium to form azo dye. The second method (Method B) is based on Schiff’s base formation reaction between MCP and 4-hydroxybenzaldehyde (HBD). The last method (Method C) is based on the formation of colored ion–pair complex from the reaction of MCP with Eosin Y (ESN). Beer’s law was obeyed in the concentration range of 1.35-40.37, 1.01-5.05 and 1.01-10.09 μg/mL MCP at 426, 386 and 543 nm with molar absorptivity of 1.51×104 L mol-1 cm-1, 2.10×104 L mol-1 cm-1 and 3.34×104 L mol-1 cm-1 for Method A, Method B and Method C, respectively. The proposed methods were successfully applied to the determination of MCP in pharmaceutical preparations without any interference from common excipients.