alexa Determination of Quercetin a Biomarker in Hepatoprotect
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Determination of Quercetin a Biomarker in Hepatoprotective Polyherbal Formulation through High Performance Thin Layer Chromatography

Arti Gupta1*, Navin R Sheth2, Sonia Pandey1 and Jitendra Singh Yadav3

1Maliba Pharmacy College, UkaTarsadia University, Bardoli, Gujarat, India

2Department of Pharmacognosy, Saurashtra University, Rajkot, Gujarat, India

3Vidyabharati Trust College of Pharmacy, Umrakh, Gujarat, India

*Corresponding Author:
Arti Gupta
Maliba Pharmacy College
UkaTarsadia University, Bardoli
Gujarat, India
Tel: +919558848242
Fax: +919558848242
E-mail: [email protected]

Received date: July 07, 2015; Accepted date: August 20, 2015; Published date: August 30, 2015

Citation: Gupta A, Sheth NR, Pandey S, Yadav LS (2015) Determination of Quercetin a Biomarker in Hepatoprotective Polyherbal Formulation through High Performance Thin Layer Chromatography. J Chromatogr Sep Tech 6:285. doi:10.4172/2157-7064.1000285

Copyright: © 2015 Gupta A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: Quercetin was determined in bioactive fractions of Ocimum gratissimum, Butea monosperma, Bauhinia variegate and polyherbal hepatoprotective formulation by HPTLC method. Methods: Polyherbal hepatoprotective formulation was developed by using five bioactive fractionated extracts of three plants namely Butea monosperma, Bauhinia variegate and O. gratissimum. All three plants contain quercetin. Chromatographic separation was performed on aluminium foil plates coated with 200 μm silica gel 60F254 Linear ascending development with toluene:ethyl acetate:formic acid, 5:4:0.1 (v/v/v) was performed at room temperature (25 ± 2°C) in a twin-trough glass chamber saturated with mobile phase vapor. Compact bands (Rf=0.38) were obtained for quercetin. Spectro densitometric scanning was performed in fluorescence mode at 380 nm. The method was validated for precision, recovery, specificity, detection and quantification limits. Results: Linear regression analysis of the calibration plots showed a good linear relationship (R²=0.9843 ± 0.0001) between peak area and concentration in the range 0.5-2.5 μg/band, respectively. The limits of detection and quantification were 0.089 and 0.26 μ/band. The recovery of the method was 97.33-99.11%. Conclusion: The above method was a rapid and cost effective quality-control tool for routine analysis of quercetin in herbal extracts and in pharmaceutical dosage form.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords