Determination of Quercetin a Biomarker in Hepatoprotective Polyherbal Formulation through High Performance Thin Layer Chromatography
- *Corresponding Author:
- Arti Gupta
Maliba Pharmacy College
UkaTarsadia University, Bardoli
E-mail: [email protected]
Received date: July 07, 2015; Accepted date: August 20, 2015; Published date: August 30, 2015
Citation: Gupta A, Sheth NR, Pandey S, Yadav LS (2015) Determination of Quercetin a Biomarker in Hepatoprotective Polyherbal Formulation through High Performance Thin Layer Chromatography. J Chromatogr Sep Tech 6:285. doi:10.4172/2157-7064.1000285
Copyright: © 2015 Gupta A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Quercetin was determined in bioactive fractions of Ocimum gratissimum, Butea monosperma, Bauhinia variegate and polyherbal hepatoprotective formulation by HPTLC method. Methods: Polyherbal hepatoprotective formulation was developed by using five bioactive fractionated extracts of three plants namely Butea monosperma, Bauhinia variegate and O. gratissimum. All three plants contain quercetin. Chromatographic separation was performed on aluminium foil plates coated with 200 μm silica gel 60F254 Linear ascending development with toluene:ethyl acetate:formic acid, 5:4:0.1 (v/v/v) was performed at room temperature (25 ± 2°C) in a twin-trough glass chamber saturated with mobile phase vapor. Compact bands (Rf=0.38) were obtained for quercetin. Spectro densitometric scanning was performed in fluorescence mode at 380 nm. The method was validated for precision, recovery, specificity, detection and quantification limits. Results: Linear regression analysis of the calibration plots showed a good linear relationship (R²=0.9843 ± 0.0001) between peak area and concentration in the range 0.5-2.5 μg/band, respectively. The limits of detection and quantification were 0.089 and 0.26 μ/band. The recovery of the method was 97.33-99.11%. Conclusion: The above method was a rapid and cost effective quality-control tool for routine analysis of quercetin in herbal extracts and in pharmaceutical dosage form.