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Determination of Rocuronium in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Pharmacokinetics in Patients | OMICS International | Abstract
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
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Editorial

Determination of Rocuronium in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Pharmacokinetics in Patients

Yuan Guiyan, Zhang Rui, Wang Benjie, Wei Chunmin, Liu Xiaoyan, Zhao Wenjing and Guo Ruichen*

Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, Jinan Shandong, China

*Corresponding Author:
Guo Ruichen
Institute of Clinical Pharmacology
Qilu Hospital of Shandong University
Jinan Shandong, China
Tel: (0351)82169636
Fax: (0531)86109975
E-mail: [email protected]

Received Date: October 23, 2012; Accepted Date: October 27, 2012; Published Date: October 31, 2012

Citation: Guiyan Y, Rui Z, Benjie W, Chunmin W, Xiaoyan L, et al. (2012) Determination of Rocuronium in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Pharmacokinetics in Patients. J Bioequiv Availab 4: xxix-xxx. doi: 10.4172/jbb.10000e23

Copyright: © 2012 Guiyan Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A sensitive and selective high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method was developed and validated for the determination of rocuronium in human plasma. The plasma samples were separated on an Agilent SB-C18 column (150×2.1 mm, 3.5 μm) with a mobile phase consisted of 20 mM ammonium acetate/methanol/ acetonitrile (20/40/40, v/v/v) at a flow rate of 0.7 mL·min-1. Electrospray ionization (ESI) source was applied and operated in the positive ion mode. Multiple Reaction Monitoring (MRM) modes with the transitions of m/z 529.3→487.3 (rocuronium) and 321.0→275.0 (IS) was used. A good linearity was obtained in the concentration range of 5~3000 μg·L-1 (r=0.9976, n=5). The inter- and intra-day precision (RSD) were less than 8.1%. The extraction recoveries were 92.0~92.6%. Rocuronium in plasma was stable when frozen at -20ºC for 24 hours and seven days, and also stable after two freeze-thawing cycles. The method is simple, quick, sensitive, reproducible and can be used for the pharmacokinetic and bioequivalence study of rocuronium.

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