Determination of Rocuronium in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Pharmacokinetics in Patients
Yuan Guiyan, Zhang Rui, Wang Benjie, Wei Chunmin, Liu Xiaoyan, Zhao Wenjing and Guo Ruichen*
Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, Jinan Shandong, China
- *Corresponding Author:
- Guo Ruichen
Institute of Clinical Pharmacology
Qilu Hospital of Shandong University
Jinan Shandong, China
E-mail: [email protected]
Received Date: October 23, 2012; Accepted Date: October 27, 2012; Published Date: October 31, 2012
Citation: Guiyan Y, Rui Z, Benjie W, Chunmin W, Xiaoyan L, et al. (2012) Determination of Rocuronium in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Pharmacokinetics in Patients. J Bioequiv Availab 4: xxix-xxx. doi: 10.4172/jbb.10000e23
Copyright: © 2012 Guiyan Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A sensitive and selective high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method was developed and validated for the determination of rocuronium in human plasma. The plasma samples were separated on an Agilent SB-C18 column (150×2.1 mm, 3.5 μm) with a mobile phase consisted of 20 mM ammonium acetate/methanol/ acetonitrile (20/40/40, v/v/v) at a flow rate of 0.7 mL·min-1. Electrospray ionization (ESI) source was applied and operated in the positive ion mode. Multiple Reaction Monitoring (MRM) modes with the transitions of m/z 529.3→487.3 (rocuronium) and 321.0→275.0 (IS) was used. A good linearity was obtained in the concentration range of 5~3000 μg·L-1 (r=0.9976, n=5). The inter- and intra-day precision (RSD) were less than 8.1%. The extraction recoveries were 92.0~92.6%. Rocuronium in plasma was stable when frozen at -20ºC for 24 hours and seven days, and also stable after two freeze-thawing cycles. The method is simple, quick, sensitive, reproducible and can be used for the pharmacokinetic and bioequivalence study of rocuronium.