Determination of the Concentration of Polyamines with SPR-based Immune Biosensor for Early Diagnostics of Breast CancerMaksym P1, Starodub N2* and Bilko N3
- Nikolay Starodub
Department of Microbiology and Biotechnology
National University of Environmental Sciences
E-mail: [email protected]
Received Date: November 16, 2016 Accepted Date:November 24, 2016 Published Date: December 04, 2016
Citation: Maksym P, Starodub N, Bilko N (2016) Determination of the Concentration of Polyamines with SPR-based Immune Biosensor for Early Diagnostics of Breast Cancer. J Biosens Bioelectron 7: 227. doi: 10.4172/2155-6210.1000227
Copyright: © 2016 Maksym P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. The method can detect the studied polyamines spermine and spermidine in concentration less than 10 ng in 1 mL, and with increasing concentrations a statistical probability of the analysis' result rise sharply. Moreover, the dependence of sensitivity of biosensor response from the concentration of polyamines is in the range of 10-100 ng /mL.