Determination of the Endocrine Disrupter Bisphenoi-A in the Blood of Uremia Patients Treated by DialysisH. Shintani1* and F. Hayashi2
- *Corresponding Author:
- Hideharu Shintani
1-10-60, Minamidaira, Hino
Tokyo 191-0041 Japan
E-mail: [email protected]
Received Date: September 12, 2011; Accepted Date: September 30, 2011; Published Date: October 03, 2011
Citation: Shintani H, Hayashi F (2011) Determination of the Endocrine Disrupter Bisphenoi-A in the Blood of Uremia Patients Treated by Dialysis. Pharm Anal Acta S11:001. doi: 10.4172/2153-2435.S11-001
Copyright: © 2011 Shintani H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Automated solid-phase extraction (SPE) then high-performance liquid chromatography have been studied for determination of the endocrine disrupter bisphenoi-A (BPA) in blood. Electro chemical detection was used for selective and sensitive detection of BPA. Determination of BPA in the blood of uremia patients treated by dialysis has not yet been reported. Acidified blood and acidified SPE eluent were used to suppress the ionization of BPA and thus retain the compound on a C-18 column. Because artificial dialysis can be performed for periods longer than 40 years, for reasons of safety the amount of BPA migrating from the artificial dialyzer into the blood of a uremia patient was compared for different sterilization methods and for different artificial dialyzers manufactured from different materials.