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ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

Determination of Thiomersal, Lidocaine and Phenylepherine in their Ternary Mixture

Nada S Abdelwahab1, Nouruddin WA1, Hamed M EL Fatatry2 and Osman WM2*

1Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Bani-sueif University -Bani-sueif, Egypt

2Pharmaceutical Analytical Chemistry Department, Faculty of pharmacy, Misr University for Science & Technology (MUST), Egypt

*Corresponding Author:
Osman WM ,
Pharmaceutical Analytical Chemistry
Department, Faculty of Pharmacy, Bani-sueif University-Bani-sueif, Egypt,
E-mail: [email protected]

Received Date: July 30, 2013; Accepted Date: August 27, 2013; Published Date: August 30, 2013

Citation: Abdelwahab NS, Nouruddin WA, Fatatry HMEL, Osman WM (2013) Determination of Thiomersal, Lidocaine and Phenylepherine in their Ternary Mixture. J Chromatograph Separat Techniq 4:199. doi:10.4172/2157-7064.1000199

Copyright: © 2013 Abdelwahab NS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

This work is concerned with the development and validation of two accurate, precise and specific spectrophotometric and chromatographic methods for analysis of Phenylepherine (PE), Lidocaine (LC) and Thiomersal (TM) in their ternary mixture and in Chromoderm® solution. The developed spectrophotometric method is the mean centering of ratio spectra (MCR) spectrophotometric method that depends on using the mean centered ratio spectra in two successive steps which eliminates the derivative steps and therefore the signal to noise ratio is enhanced. On the other hand, the developed chromatographic method isocratic RP-HPLC method that depends chromatographic separation on Zorbax C18 column using a mobile phase consisting phosphate buffer: acetonitrile: triethylamine (40:60:0.1, by volume pH=6 with orthophosphoric acid). The flow rate was 0.6 mL min-1 and the eluent was monitored at 245 nm. Factors affecting the developed methods were studied and optimized. Moreover, they have been validated as per ICH guidelines. The developed methods have been successfully applied for determination of PE, LC and TM in different laboratory mixtures and in solution dosage form. Also, Statistical analysis of the results of the two developed methods with each other using F and student’s t tests showed no significant difference

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