Determination of Ti from TiO2 Nanoparticles in Biological Materials by Different ICP-MS Instruments: Method Validation and Applications
- *Corresponding Author:
- Thierry Guérin
Anses, Food Safety Laboratory–Maisons-Alfort
Chemical Food Contaminants Department
Metallic Trace Elements and Minerals Unit, 14
rue Pierre et Marie Curie, F-94706 Maisons-Alfort, France
E-mail: [email protected]
Received Date: January 14, 2015; Accepted Date: February 09, 2015; Published Date: February 19, 2015
Citation: Nia Y, Millour S, Noël L, Krystek P, Jong W, et al. (2015) Determination of Ti from TiO2 Nanoparticles in Biological Materials by Different ICP-MS Instruments: Method Validation and Applications. J Nanomed Nanotechnol 6:269. doi: 10.4172/2157-7439.1000269
Copyright: © 2015 Nia Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The accurate determination of nanoparticles (NPs) content in biological samples is of great interest for studying its potential impacts on health. This paper describes a method validation process for Ti determination from TiO2 NPs in biological matrixes using a closed-vessel microwave digestion with a mixture of 3 mL of HNO3 and 0.4 mL of HF and a detection by quadrupole inductively coupled plasma-mass spectrometry (Q-ICP-MS), in both standard and collision/ reaction cell (CCT) modes. As no suitable certified reference materials are available, several internal reference materials (IRMs) were prepared precisely and they were used for the optimisation and the analytical quality assurance of the method. Several criteria such as linearity, limits of quantification (LOQ), precision under repeatability conditions and intermediate precision reproducibility were evaluated. Furthermore, the method was compared on different Q-ICP-MS devices and on a high resolution (HR)-ICP-MS using the prepared IRMs and various samples of rat tissues exposed to two different sizes of TiO2 NPs. The results demonstrated that the Q-ICP-MS method evaluated permitted an accurate determination of Ti present as TiO2 NPs in both standard and CCT modes, with a greater accuracy in CCT mode at low concentration levels near the LOQ of 0.10 mg kg-1.