alexa Developing Rapid and Reliable Regeneration System Using Cotyledonary-Node Method in Indian Soybean Genotypes (Glycine Max L.)
ISSN ONLINE(2319-8753)PRINT(2347-6710)

International Journal of Innovative Research in Science, Engineering and Technology
Open Access

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Research Article

Developing Rapid and Reliable Regeneration System Using Cotyledonary-Node Method in Indian Soybean Genotypes (Glycine Max L.)

Alkesh Hada1, Amit Kumar Gupta1, Theboral Jeevaraj2, Markandan Manickavasagam2, Andy Ganapathi2, Monica Jolly1 , Archana Sachdev1
  1. Ph.D scholar, Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, India1, SRF1, Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, India
  2. Ph.D scholar, Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
  3. Assistance Professor, Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
  4. Professor, Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
  5. Technical Officer, Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, India
  6. Professor Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, India
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Abstract

Regeneration through mature cotyledonary node has set rapid regeneration of plants directly from explants which is more time-saving and presented as an effective strategy so we have developed regeneration protocol through single shoot using cotyledonary node a rapid and efficient protocol for three Indian soybean cultivars. Two explants were collected from single cotyledonary node and cultured on medium containing N6-benzylaminopurine (BAP) for germination, BAP and indole-3-butyric acid (IBA) for shoot induction, Gibberellic acid (GA3) for shoot elongation and IBA for rooting of explants. The best combination of hormones for all genotypes were obtained as germination of seeds on half B5 medium supplemented with 1.0 mgl-1 of BAP, shoot induction on full B5 medium having BAP 1.0 mgl-1 and IBA 0.2 mgl-1 and shoot elongation on GA3 0.75 mgl-1 in full MS medium. Under these conditions, the plantlets could be raised within 40-45 days. It was observed that selection of proper medium for regeneration of soybean can overcome genotype associated problems. This regeneration system can be used for soybean transformation

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