Development and Clinical Trial of a Novel DNA Vaccine as Immunotherapy during Canine Leishmaniasis
Laura Manna1*, Ilaria Michela Piras2, Valentina Ciprì1, Alberto Alberti2, Italia Della Peruta1, Carlo Maria Del Pizzo1, Nicoletta Gammarano1, Elisabetta Coradduzza2, Carla Cacciotto2, Marco Pittau2, Angelo Elio Gravino1 and Bernardo Chessa2
- *Corresponding Author:
- Laura Manna
Dipartimento di Scienze Cliniche Veterinarie
Università di Napoli Federico II
Via F. Delpino, n.1, 80137 Napoli, Italy
E-mail: [email protected]
Received date: September 30, 2012; Accepted date: October 17, 2012; Published date: October 20, 2012
Citation: Manna L, Piras IM, Ciprì V, Alberti A, Peruta ID, et al.(2012) Development and Clinical Trial of a Novel DNA Vaccine as Immunotherapy during Canine Leishmaniasis. J Vaccines Vaccin 3:155. doi:10.4172/2157-7560.1000155
Copyright: © 2012 Manna L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Visceral Leishmaniasis (VL) is a zoonotic disease caused by Leishmaniainfantum in the Mediterranean area. Dogs are the main reservoir of Leishmaniainf antum parasites. Disease management represents a serious problem, since anti-Leishmania drugs have limited efficacy in both symptomatic and asymptomatic dogs, which are infective to phlebotomine vectors. In many tropical and sub-tropical countries the development of a safe and easily-available vaccine has high priority. DNA vaccines represent one of the most recent innovations in the field of immunization.
Findings: This study aimed to evaluate the effect of a DNA vaccine based on two Leishmania antigens (Cpb1, PO) in leishmaniotic dogs. Twelve leishmaniotic dogs from a Leishmaniasis-endemic area (Naples, Italy) received three consecutive injections of DNA vaccine at 15-days intervals. Another group of five leishmaniotic dogs received the same amount of pVAX-1 without the coding sequences of Leishmania antigens. Leishmania DNA load, INFγ, IL-4 mRNA expression levels and clinical parameters were tested before and after the therapy, every 3 months for a period of 12 months.
Analysis of the data in the vaccinated dogs showed: i) a decrease Leishmania DNA load in lymph node samples, ii) an increase of INFγ and IL-4 mRNA expression levels in PBMC samples. All vaccinated dogs also showed an improvement in the clinical symptoms.
Conclusion: Our results show that the vaccine developed in this study may represent a useful tool in the treatment of leishmaniotic dogs. However, since the duration of positive effects is limited in time, further trials are needed to evaluate the effectiveness of immunotherapy alone or in association with conventional therapy.