Development and Validation of a Gas Chromatography-Mass Spectrometry Method for the Determination of PCBs in Transformer Oil Samples-Application on Real Samples from BotswanaSaidy Motladiile1*, Habauka M Kwaambwa2 and Kwenga Sichilongo3
- *Corresponding Author:
- Saidy Motladiile
Department of Waste management and Pollution Control
National Environmental Laboratory
PB BO323, Gaborone, Botswana
Tel: +267 3934479
Fax: +267 3934486
E-mail: [email protected]
Received date: December 02, 2011; Accepted date: January 03, 2012; Published date: January 06, 2012
Citation: Motladiile S, Kwaambwa HM, Sichilongo K (2011) Development and Validation of a Gas Chromatography-Mass Spectrometry Method for the Determination of PCBs in Transformer Oil Samples-Application on Real Samples from Botswana. J Chromatograph Separat Techniq 2:116. doi:10.4172/2157-7064.1000116
Copyright: © 2011 Motladiile S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A procedure in which a retention time window for the determination of polychlorinated biphenyls (PCBs) congeners including those not presented in this study but of similar molar masses in transformer oil has been developed and validated. This procedure was successfully applied in the determination of transformer oil samples from Botswana for comparison with a preliminary inventory for PCB levels in transformer oil and other receptacles suspected to contain PCBs and developed during the formulation of the Botswana National Implementation Plan (NIP) for the sound management of persistent organic pollutants (POPs). Recoveries in the developed procedure initially meant for 39 PCB congeners ranged from 44.53±0.31 to 111.93±4.75% uncorrected for bias, while linearities i.e. r 2 values ranged from 0.9624 to 0.9999 with method detection limits (MDLs) varying from 0.02 to 0.13 ppm (w/v). Application of this procedure on real samples from Botswana showed that the transformer oil contained no PCBs although the preliminary data using screening equipment and kits for indicative inventory data showed high PCB contents in some cases of as much as 456 ppm (w/v). It is proposed here that this method be adopted in the SADC region for confirmation of presence and determination of the levels of PCBs where they are detected in order to improve on the preliminary inventories that were developed during the NIP development exercise for the Stockholm Convention (SC) in these countries.