alexa Development and Validation of a Liquid Chromatographic
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Development and Validation of a Liquid Chromatographic Method for the Determination of Cefdinir Residues on Manufacturing Equipment Surfaces

Magda A Akl1*, Mona A Ahmed2 and Ahmed Ramadan1

1Chemistry Department, Faculty of Science, Mansoura University, Mansoura, Egypt

2Chemistry Department, College of Girls, Ain Shams University, Cairo, Egypt

*Corresponding Author:
Magda A Akl
Chemistry Department, Faculty of Science
Mansoura University, Mansoura, Egypt
E-mail: [email protected]

Received Date: January 31, 2013; Accepted Date: April 17, 2013; Published Date: April 20, 2013

Citation: Akl MA, Ahmed MA, Ramadan A (2013) Development and Validation of a Liquid Chromatographic Method for the Determination of Cefdinir Residues on Manufacturing Equipment Surfaces. J Chromat Separation Techniq 4:177. doi:10.4172/chromatography-separation-techniques.1000177

Copyright: © 2013 Akl MA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited



The cleaning validation procedure for the manufacturing equipment surfaces of cefdinir was done using cotton swabs moistened with the extraction solution (900 ml of water and 3.0 ml phosphoric acid and then adjusting the pH to 7.0 ± 0.05). The HPLC method was validated on a LC system using Waters (USA) Symmetry - C18 (250 mm×4.6 mm×5 μm) at 25°C in the presence of a mobile phase composed of acetonitrile: pH 7 buffer (85-15) as at flow rate of 1.0 ml/min and an injection volume of 20 μl over the concentration range 14.5-74.5 μg mL-1. UV detection was made at 254 nm. The detection limit (DL) and quantification limit (QL) were 0.7 and 2.2 μg mL-1, respectively. The intra-day and inter-day precisions, expressed as relative standard deviation (R.S.D.), were below 2.00%. The recoveries were 98.68, 101 and 102.28% for three concentration levels with an average recovery of 100.65%.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version