Development and Validation of a Liquid Chromatography - Tandem Mass Spectrometry Method for the Determination of Voriconazole and Posaconazole in Serum Samples from Patients with Invasive Mycoses
Loretta Franceschi*, Sara D’Aronco and Mario Furlanut
Chair of Pharmacology, Institute of Clinical Pharmacology and Toxicology, DISM, University of Udine, Udine, Italy
- *Corresponding Author:
- Dr. Loretta Franceschi
Chair of Pharmacology
Institute of Clin Pharm & Toxicol - DISM, University of Udine
Piazzale SM della Misericordia, 1533100 Udine - Italy
Tel: +39 (0)432 559833
Fax: +39 (0)432 559819
E-mail: [email protected]
Received Date: June 10, 2011; Accepted: July 23, 2011; Published Date: July 27, 2011
Citation: Franceschi L, Aronco SD, Furlanut M (2011) Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Voriconazole and Posaconazole in Serum Samples. J Bioanal Biomed 3: 092-097. doi: 10.4172/1948-593X.1000050
Copyright: © 2011 Franceschi L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Posaconazole and voriconazole, two triazole antifungal agents, are used for the prophylaxis and treatment of invasive mycoses in patients with acute myeloid leukaemia and/or immunocompromised. Inter- and intra-patient variability of pharmacokinetics, drug-drug interactions, serum concentration related toxicity and success of therapy has stressed the need of frequently therapeutic drug monitoring of both drugs. Therefore, a rapid, selective and sensitive isocratic reversed-phase HPLC assay coupled with Mass spectrometry detection for quantification of posaconazole and voriconazole in serum samples has been developed.
Analytes were extracted on solid-phase cartridges (SPE) and chromatographic separation was achieved on a C8 column and detected by mass spectrometry in positive ion mode with the select ion monitoring (SIM) mode. The total chromatographic running time was 6 minutes. The method was successfully used for a pharmacokinetic study but, thanks to its rapidity and selectivity, it’s also suitable for routinarly therapeutic drug monitoring (TDM).