Development and Validation of a New Chromatographic Method for the Simultaneous Estimation of Serratiopeptidase, Aceclofenac and Paracetamol by RP-HPLC
- *Corresponding Author:
- Afroze Alam
School of Pharmaceutical Sciences
Shoolini University, Bajhol, Solan-173 229
Himachal Pradesh, India
E-mail: [email protected]
Received Date: February 18, 2017 Accepted Date: March 07, 2017 Published Date: March 13, 2017
Citation: Navneet Kumar U, Afroze A, Pradeepti C, Shailendra K, Naik KK, et al. (2017) Development and Validation of a New Chromatographic Method for the Simultaneous Estimation of Serratiopeptidase, Aceclofenac and Paracetamol by RP-HPLC. Pharm Anal Chem 3: 122. doi: 10.4172/2471-2698.1000122
Copyright: © 2017 Navneet Kumar U, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Method development, validation is an important parameter for the simultaneous estimation of Serratiopeptidase (SERA), Aceclofenac (ACE) and Paracetamol (PCM) by RP-HPLC, is supposed to be a costly and tedious process. The present study revealed using cheap and cost effective solvent system for the simultaneous estimation of Serratiopeptidase (SERA), Aceclofenac (ACE) and Paracetamol (PCM).
Objective: Development and validation of a new chromatographic method for the simultaneous estimation of Serratiopeptidase (SERA), Aceclofenac (ACE) and Paracetamol (PCM) by RP-HPLC of marketed formulations.
Methods: Simultaneous determination of SERA, ACE and PCM were carried out by RP-HPLC at the wavelength 327 nm, flow rate 0.4 mL/min, and the mobile phase used was water: methanol in the ratio (50:50 v/v). Further validation parameters such as system suitability, linearity, accuracy, precision, specificity, LOD, LOQ and robustness were taken into account to carry out the validation of the method.
Results: Absorbance maxima for the simultaneous determination were selected by the UV spectrophotometer and that was found to be 327 nm in methanol and water. During the process of RP-HPLC, the linearity was obtained in the concentration range of 2-10 μg/mL for SERA, 100-500 μg/mL for ACE and 20-100 μg/mL for PCM. Correlation coefficient (r) for SERA, ACE and PCM in methanol and water was found to be 0.9817, 0.991 and 0.9949 respectively.
Conclusion: The RP-HPLC method was simple, accurate, precise, and rapid and can be used for the simultaneous determination of SERA, ACE and PCM in bulk and pharmaceutical dosage form. The method is also economical as RP-HPLC grade water methanol in a ratio of (50:50) was used to achieve all the validation parameters.