alexa Development and Validation of a New Chromatographic Met
ISSN: 2471-2698

Pharmaceutical Analytical Chemistry: Open Access
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Research Article

Development and Validation of a New Chromatographic Method for the Simultaneous Estimation of Serratiopeptidase, Aceclofenac and Paracetamol by RP-HPLC

Navneet Kumar U1, Afroze A1,2*, Pradeepti C1, Shailendra K4, Naik KK3, Sharma M1, Swati P1, Sunil T1 and Sameer S1

1School of Pharmaceutical Sciences, Shoolini University, Bajhol, Solan, Himachal Pradesh, India

2Narayan Institute of Pharmacy, Jamuhar, Sasaram, India

3Nandha College of Pharmacy, Erode, Tamil Nadu, India

4Government Pharmacy College, Agam Kuan, Patna, Bihar, India

*Corresponding Author:
Afroze Alam
School of Pharmaceutical Sciences
Shoolini University, Bajhol, Solan-173 229
Himachal Pradesh, India
Tel: +919805185732/+918091974886
E-mail: [email protected]

Received Date: February 18, 2017 Accepted Date: March 07, 2017 Published Date: March 13, 2017

Citation: Navneet Kumar U, Afroze A, Pradeepti C, Shailendra K, Naik KK, et al. (2017) Development and Validation of a New Chromatographic Method for the Simultaneous Estimation of Serratiopeptidase, Aceclofenac and Paracetamol by RP-HPLC. Pharm Anal Chem 3: 122. doi: 10.4172/2471-2698.1000122

Copyright: © 2017 Navneet Kumar U, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Background: Method development, validation is an important parameter for the simultaneous estimation of Serratiopeptidase (SERA), Aceclofenac (ACE) and Paracetamol (PCM) by RP-HPLC, is supposed to be a costly and tedious process. The present study revealed using cheap and cost effective solvent system for the simultaneous estimation of Serratiopeptidase (SERA), Aceclofenac (ACE) and Paracetamol (PCM).

Objective: Development and validation of a new chromatographic method for the simultaneous estimation of Serratiopeptidase (SERA), Aceclofenac (ACE) and Paracetamol (PCM) by RP-HPLC of marketed formulations.

Methods: Simultaneous determination of SERA, ACE and PCM were carried out by RP-HPLC at the wavelength 327 nm, flow rate 0.4 mL/min, and the mobile phase used was water: methanol in the ratio (50:50 v/v). Further validation parameters such as system suitability, linearity, accuracy, precision, specificity, LOD, LOQ and robustness were taken into account to carry out the validation of the method.

Results: Absorbance maxima for the simultaneous determination were selected by the UV spectrophotometer and that was found to be 327 nm in methanol and water. During the process of RP-HPLC, the linearity was obtained in the concentration range of 2-10 μg/mL for SERA, 100-500 μg/mL for ACE and 20-100 μg/mL for PCM. Correlation coefficient (r) for SERA, ACE and PCM in methanol and water was found to be 0.9817, 0.991 and 0.9949 respectively.

Conclusion: The RP-HPLC method was simple, accurate, precise, and rapid and can be used for the simultaneous determination of SERA, ACE and PCM in bulk and pharmaceutical dosage form. The method is also economical as RP-HPLC grade water methanol in a ratio of (50:50) was used to achieve all the validation parameters.


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