alexa Development and Validation of Analytical Method for Qualitative and Quantitative Determination of Glibenclamide in Different Brands of Tablet Dosage form Using UV-Visible Spectroscopy | Abstract
ISSN: 1747-0862

Journal of Molecular and Genetic Medicine
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Research Article

Development and Validation of Analytical Method for Qualitative and Quantitative Determination of Glibenclamide in Different Brands of Tablet Dosage form Using UV-Visible Spectroscopy

Abida Bilal1, Kanwal Rehman2, Muhammad Sajid Hamid Akash2,3#, Khalid Hussain1, Muhammad Ibrahim4 and Syed Saeedul Hussan1

1University College of Pharmacy, University of the Punjab, Lahore, Pakistan

2Institute of Pharmacology, Toxicology and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China

3College of Pharmacy, Government College University Faisalabad, Faisalabad, Pakistan

4Department of Applied Chemistry, Government College University Faisalabad, Faisalabad, Pakistan

*Corresponding Author:
Muhammad Sajid Hamid Akash
College of Pharmacy
Government College University Faisalabad
Faisalabad, Pakistan
E-mail:[email protected]

Received date: September 04, 2013; Accepted date: October 11, 2013; Published date: October 15, 2013

Citation: Bilal A, Rehman K, Akash MSH, Hussain K, Ibrahim M, et al. (2013) Development and Validation of Analytical Method for Qualitative and Quantitative Determination of Glibenclamide in Different Brands of Tablet Dosage form Using UV-Visible Spectroscopy. J Mol Genet Med 7:80. doi: 10.4172/1747-0862.1000080

Copyright: © 2013 Bilal A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Abstract

This paper describes a methodology to develop a new method for qualitative and quantitative determination of glibenclamide (GLB) in three brands of GLB tablets. We validated the developed method for linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) according to the guidelines of the International Conference on Harmonization. Finally, we estimated the qualitative and quantitative amount of GLB in three brands of GLB tablets using validated method. A non significant difference was observed in the dissolution profiles of GLB in these tablets. Validation of developed method showed that a linear relationship (r2>0.999) was observed at maximum absorbance (λmax) at 229.5 nm with concentration range of 3-15 μg/ml GLB. Accuracy of developed method was found to be within the limit of 95-105%. The percent relative standard deviation (%RSD) and percent relative error (%RE) for precision was found to be <3%. In three brands of GLB tablets, values of LOD and LOQ were 10 ng/ml and 35 ng/ml respectively. We also found that the amount of GLB in each tablet corresponded to the requirements of 95-105% of the label claimed in tablet. From the results of validation of developed method, it concluded that developed method showed satisfactory linearity, precision and accuracy for analysis of active ingredients of commercially available pharmaceutical products.

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