Development and Validation of High-Performance Liquid Chromatography - Diode Array Detector Method for the Determination of Tramadol in Human SalivaAbdel-Aziz Y. El-Sayed1*, Khaled M. Mohamed2, Maha A.Hilal3, Soheir A.Mohamed3, Khaled E. Aboul-Hagag3and Ahmed Y. Nasser4
- *Corresponding Author:
- Dr. Abdel-Aziz Y. El-Sayed
Chemistry Department, Faculty of Science
Al-Azhar University (Assiut Branch), Egypt
E-mail: [email protected] yahoo.com
Received date: August 29, 2011; Accepted date: October 11, 2011; Published date: October 15, 2011
Citation: El-Sayed AAY, Mohamed KM, Hilal MA, Mohamed SA, Aboul-Hagag KE, et al. (2011) Development and Validation of High-Performance Liquid Chromatography-Diode Array Detector Method for the Determination of Tramadol in Human Saliva. J Chromatograph Separat Techniq 2:114. doi:10.4172/2157-7064.1000114
Copyright: © 2011 El-Sayed AAY, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A high-performance liquid chromatography–diode array detector method (HPLC-DAD) was developed and validated for the determination of tramadol in human saliva. Samples of saliva were prepared utilizing liquid–liquid extraction with hexane-ethyl acetate (4:1, v/v). Propranolol was used as an internal standard (IS). The linear range and average recovery of tramadol were 0.25–4.00 μg/mL and 94.70%, respectively. The intra- and inter-assay precisions at low, intermediate and high concentrations were within 1.40–9.79 % and 0.77–3.98%, respectively. Intra- and inter-assay accuracies were within 98.50–107.13% and 100.44–103.89%, respectively. The proposed method was successfully applied to determine tramadol in saliva concentrations in three healthy volunteers for 24 hours after administration of 100 mg oral doses of tramadol.