alexa Development and Validation of HPLC Method for Simultane
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

Development and Validation of HPLC Method for Simultaneous Estimation of Brimonidine Tartrate and Timolol Maleate in Bulk and Pharmaceutical Dosage Form

Abdullah A Elshanawane1, Lobna M Abdelaziz1, Mustafa S Mohram2 and Hany M Hafez2*
1Medicinal Chemistry Department, Zagazig University, Zagazig, Egypt
2Quality control department, EIPICO, Egypt
Corresponding Author : Hany M. Hafez
Bachelor degree of Pharmaceutical Science
Zagazig University, Zagazig, Egypt Quality control department
EIPICO, 10th Ramadan, Egypt
Tel: 020113231458
E-mail: [email protected]
Received April 29, 2014; Accepted June 30, 2014; Published July 17, 2014
Citation: Elshanawane AA, Abdelaziz LM, Mohram MS, Hafez HM (2014) Development and Validation of HPLC Method for Simultaneous Estimation of Brimonidine Tartrate and Timolol Maleate in Bulk and Pharmaceutical Dosage Form. J Chromatograph Separat Techniq 5:230. doi:10.4172/2157-7064.1000230
Copyright: © 2014 Elshanawane AA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Brimonidine tartrate and Timolol Maleate are used in treatment of glaucoma by decreasing intra ocular pressure. A validated HPLC method was developed for the assay of them. The method was performed on BDS HYPERSIL Cyano column (250×4.6 mm, 5 μ) and the mobile phase consisted of Ammonium acetate (pH 5.0, 0.01 M) - Methanol (40:60, V/V) which pumped at a flow rate equals to 1.5 ml/min at ambient temperature. 20 μl of drugs sample solutions were monitored at two fixed wavelengths (lambda=254.0 nm for Brimonidine Tartrate and 300.0 nm for Timolol Maleate). The proposed method was validated in terms of linearity, accuracy, precision and limits of detection and quantitation according to ICH.


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