alexa Development and Validation of RP- HPLC Method for Simul
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Development and Validation of RP- HPLC Method for Simultaneous Determination of Ascorbic Acid and Salicylamide in their Binary Mixtures: Application to Combined Tablets

M. Sharaf El-Din, M Eid and A M Zeid*

Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516, Mansoura, Egypt

*Corresponding Author:
A. M. Zeid
Department of Analytical Chemistry
Faculty of Pharmacy
University of Mansoura
35516, Mansoura, Egypt
Tel: +20502247496
E-mail: [email protected]

Received date: July 22, 2012; Accepted date: September 05, 2012; Published date: September 09, 2012

Citation: El-Din MS, Eid M, Zeid AM (2012) Development and Validation of RPHPLC Method for Simultaneous Determination of Ascorbic Acid and Salicylamide in their Binary Mixtures: Application to Combined Tablets. J Chromat Separation Techniq 3:137. doi:10.4172/2157-7064.1000137

Copyright: © 2012 El-Din MS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



A new simple, rapid and sensitive reversed-phase liquid chromatographic method was developed and validated for the simultaneous determination of ascorbic acid (ASC) and salicylamide (SAL) in their combined dosage form. The analysis was carried out on CLC Shim-pack C8 column (250 x 4.6 mm, 5 μm particle size) using a mobile phase consisting of methanol: 0.03 M phosphate buffer mixture (55: 45, v/v) of pH 4.0. The mobile phase was pumped at a flow rate of 1 mL/min with ultraviolet detection at 255 nm. The selectivity, linearity of calibration, accuracy, intra and inter day precision and recovery were examined as parts of the method validation. The concentration– response relationship was linear over a concentration range of 0.50-10.00 and 5.00-50.00 μg/mL for ASC and SAL, respectively with limits of detection of 0.048 and 0.676 μg/mL. The proposed method was applied for the simultaneous determination of the two studied drugs in their combined tablets with average recoveries of 100.04 ± 0.75% and 100.11 ± 1.04% for ASC and SAL, respectively. The results were favorably compared to those obtained by the comparison methods.


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