alexa Development and Validation of RP-HPLC Method for Simult
ISSN : 2153-2435

Pharmaceutica Analytica Acta
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Research Article

Development and Validation of RP-HPLC Method for Simultaneous Determination of Diclofenac Sodium and Eperisone Hydrochloride in Pharmaceutical Dosage Form

Divya A1, Vishwanadham Y2* and Mounika1

1Department of Pharmaceutical Analysis and QA, Vishnu Institute of Pharmaceutical Education and Research, Telangana 502313, India

2Department of Pharmaceutical Chemistry, Vishnu Institute of Pharmaceutical Education and Research, Telangana 502313, India

*Corresponding Author:
Vishwanadham Y
Department of Pharmaceutical Chemistry
Vishnu Institute of Pharmaceutical Education and Research
Narsapur, Medak-502313
Telangana, India
Tel: 040-64634235
E-mail: [email protected]

Received Date: May 17, 2017; Accepted Date: June 21, 2017; Published Date: June 26, 2017

Citation: Divya A, Vishwanadham Y, Mounika (2017) Development and Validation of RP-HPLC Method for Simultaneous Determination of Diclofenac Sodium and Eperisone Hydrochloride in Pharmaceutical Dosage Form. Pharm Anal Acta 8:552. doi: 10.4172/2153-2435.1000552

Copyright: © 2017 Divya A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The RP-HPLC (Reversed-Phase High-Performance Liquid Chromatography) method was developed for the simultaneous determination of Eperisone Hydrochloride and Diclofenac Sodium in capsule dosage form. After that optimization good chromatographic separation was achieved by Isocratic mode with a mixture of Acetonitrile: Phosphate buffer pH-5.8 in the ratio 55:45 v/v as the mobile phase and detection wavelength of 225 nm. The drud retention times for Eperisone Hydrochloride and Diclofenac Sodium found to be 3.143 min and 4.753 min respectively. The linearity, this method was found in the concentration range of 90-210 μg/ml for Eperisone Hydrochloride and 60-140 μg/ml for Diclofenac Sodium. The LOD and LOQ for Eperisone Hydrochloride were found to be 1.55 and 4.71 μg/ml respectively. The LOD and LOQ for Diclofenac Sodium were found to be 2.08 and 6.31 μg/ml respectively. This method was found to be good as the percentage recovery for Eperisone Hydrochloride and Diclofenac Sodium were found to be 100.86 and 99.81 respectively, which indicates that the proposed method is highly accurate. The specificity of the method shows good correlation between retention times of standard with the drug so, the sample without interference from excipients of capsule dosage form.

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