Development and Validation of Scar Marker for Stem Rust Resistance Gene Sr26 in Wheat (Triticum aestivum L.)Ruchi Rai*, Das BK and Bhagwat SG
Nuclear Agriculture and Biotechnology Division; Bhabha Atomic Research Centre, India
- Corresponding Author:
- Ruchi Rai
Nuclear Agriculture and Biotechnology Division
Bhabha Atomic Research Centre, Mumbai-400085, India
E-mail: [email protected]
Received date: April 24, 2017; Accepted date: May 16, 2017; Published date: May 24, 2017
Citation: Rai R, Das BK, Bhagwat SG (2017) Development and Validation of Scar Marker for Stem Rust Resistance Gene Sr26 in Wheat (Triticum aestivum L.). Biol Syst Open Access 6:181. doi:10.4172/2329-6577.1000181
Copyright: © 2017 Rai R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
The stem rust resistance gene Sr26 imparts resistance to all the virulent pathotypes of stem rust (Puccinia graminis f. sp. tritici) including a new race of pathogen named TTKSK (syn.Ug99). Wheat genotypes including noncarrier Kalyan Sona (-Sr26) and carrier Kite (+Sr26) of the Sr26 gene were analysed using polymerase chain reactions and a RAPD marker OPAE07620 was found to link with the desired trait. Linkage between the marker OPAE07620 and rust resistance phenotype was confirmed by analyzing F2 population obtained from a cross between a resistant and susceptible genotype. The Random Amplified Polymorphic DNA (RAPD) marker was converted into sequence characterized amplified region (SCAR) marker SCOPAE07620. The marker was validated in another segregating population and 45 wheat genotypes including carrier and non-carriers of Sr26 gene. The marker developed in this study can be used for pyramiding of the Sr26 gene with other major resistance genes that are effective against the TTKSK lineage (Ug99) which will help in attaining durable resistance against a disease that poses a serious threat to global wheat production.