alexa Development and Validation of Simultaneous Equation Method for the Estimation of Metformin amd Sitagliptin by UV Spectroscopy
e-ISSN:2320-1215 p-ISSN: 2322-0112

Research & Reviews in Pharmacy and Pharmaceutical Sciences
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Research Article

Development and Validation of Simultaneous Equation Method for the Estimation of Metformin amd Sitagliptin by UV Spectroscopy

Sujani PV1*, SS Phanindra1, P Anasuya2, Y Padamanabha Reddy3 and N Devanna4

1Department of Pharmaceutical Analysis, Procadence Institute of Pharmaceutical Sciences, Rimmanaguda, Medak (dt), Andra Pradesh, India.

2Assistant Manager, Department of Pharmacokinetics, Mylan Laboratories, Hyderabad, Andhra Pradesh, India.

3Principal, RIPER, Anantapur, Andhra Pradesh, India.

4Associate Professor of Chemistry, JNTU-College of Engineering, Anantapur, Andhra Pradesh, India.

*Corresponding Author:
Sujani PV
Department of Pharmaceutical Analysis
Procadence Institute of Pharmaceutical Sciences
Rimmanaguda, Medak (dt), Andra Pradesh, India
Mobile: +91 9642435074

Received: 29/03/2013 Accepted: 12/04/2013



A simple, sensitive, rapid, economic, UV Spectroscopic method was developed for the estimation of Sitagliptin-Metformin in Pure and Tablet dosage forms. The linearity for Metformin was found between 200-400 μ/ml and between 20-45 μ/ml for Sitagliptin. Metformin showed the maximum absorbance at 232nm & Sitagliptin at 266nm and Validation parameters like Precision, Accuracy, and System suitability parameters were determined and examined by applying validated parameters.


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