Development and Validation of Spectrophotometric Method for Determination of Oxyfluorfen Herbicide ResiduesKhansaa Fatta Alrahman1, Abdala A Elbashir1* and Hassan El-obid Ahmed1,2
- *Corresponding Author:
- Abdala A. Elbashir
Faculty of Science
University of Khartoum
Khartoum, P.O. Box 321
E-mail: [email protected]
Received date: August 04, 2015; Accepted date: August 18, 2015; Published date: August 24, 2015
Citation: Alrahman KF, Elbashir AA, El-obid Ahmed H (2015) Development and Validation of Spectrophotometric Method for Determination of Oxyfluorfen Herbicide Residues. Med chem 5:383-387. doi: 10.4172/2161-0444.1000290
Copyright: © 2015 Alrahman KF, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Simple spectrophotometricmethod has been developed and validated for the determination of oxyfluorfen herbicide residues. The proposed method is based on the formation of an orange charge- transfer complex between oxyfluorfen pesticideas electron donor and 1,2- naphthoquinone-4-sulphonate (NQS) as electron acceptor. In basic medium pH 13.0, an orange colored product exhibiting maximum absorption peak (λ max) at 460 nm. The variables that affected the reaction such as pH, concentration and volume of NQS reagent, amount of buffer solution and reaction time were carefully studied and optimized. Under the optimum conditions, Beer’s law is obeyed in the range 0.4-4.0 μg/ mL of oxyfluorfen. The linear regression equation of the calibration curve is A=0.0906+0.2579 c (μg/mL), with a linear regression correlation coefficient of 0.9993. The molar absorptivity was 1.33 × 105 l/mol cm. The limits of detection (LOD) limits of quantification (LOQ) were found to be 0.12 μg/ mL, 0.36 μg/mL, respectively. The recovery rate is in the range of 93.50-103.00% was obtained. The proposed method has been successfully applied to the determination of oxyflourfen pesticide residues in tomato, onion and water with good accuracy and precision.