Development and Validation of Stability-Indicating RP-UPLC Method for Simultaneous Estimation of Amlodipine Besylate and Atorvastatin Calcium in Pharmaceutical Dosage Forms
- *Corresponding Author:
- Mallikarjuna S
Raghavendra Institute of Pharmaceutical
Education & Research (RIPER)
E-mail: [email protected]
Received Date: May 13, 2013; Accepted Date: June 26, 2013; Published Date: June 28, 2013
Citation: Mallikarjuna S, Ramalingam P, Sriram P, Garima J, Srinivas SK (2013) Development and Validation of Stability-Indicating RP-UPLC Method for Simultaneous Estimation of Amlodipine Besylate and Atorvastatin Calcium in Pharmaceutical Dosage Forms. J Chromatograph Separat Techniq 4:187. doi: 10.4172/2157-7064.1000187
Copyright: © 2013 Mallikarjuna S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A stability indicating Ultra Performance Liquid Chromatography (UPLC) method was developed and validated for the simultaneous determination of Atorvastatin Calcium (ASC) and Amlodipine Besylate (AMB) in tablets. The chromatographic separation was performed on acquity UPLC, Kromasil C18, 50×2.1 mm, 3.5 μm using gradient elution of acetonitrile and 0.1% v/v Triethyl amine buffer (pH 3 ± 0.05) at flow rate of 0.8 ml/min. UV (Ultra Violet) detection was performed at 240 nm. Total run time was 2.2 min within which main compounds and degradants were separated. Stability indicating capability was established by forced degradation experiments. The method was validated for accuracy, repeatability, reproducibility and robustness. Linearity, Limit of Quantification (LOQ) and Limit of Detection (LOD) was also established.