Development, Characterization and Cell Cultural Response of 3D Biocompatible Micro-Patterned Poly-ε- Caprolactone Scaffolds Designed and Fabricated Integrating Lithography and Micromolding Fabrication Techniques.
4 Interregional Research Center for Food Safety & Health (IRC-FSH), Department of Health Science, University "Magna Graecia" of Catanzaro, Complesso "Ninì Barbieri", 88021 Roccelletta di Borgia, Italy
- Corresponding Author:
- Tania Limongi
Physical Science & Engineering (PSE) Division
Biological and Environmental Sciences and Engineering (BESE) Division
King Abdullah University of Science and Technology Bldg. 2
4rd floor, Desk 4315-WS09, Desert side, Thuwal, 23955 -6900, Kingdom of Saudi Arabia
E-mail: [email protected]
Received date: October 31, 2014; Accepted date: December 09, 2014; Published date: December 12, 2014
Citation: Limongi T, Miele E, Shalabaeva V, Rocca RL, Schipani R, et al. (2015) Development, Characterization and Cell Cultural Response of 3D Biocompatible Micro-Patterned Poly-ε-Caprolactone Scaffolds Designed and Fabricated Integrating Lithography and Micromolding Fabrication Techniques. J Tissue Sci Eng 6:145. doi: 10.4172/2157-7552.1000145
Copyright: © 2015 Limongi T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Scaffold design and fabrication are very important subjects for biomaterial, tissue engineering and regenerative medicine research playing a unique role in tissue regeneration and repair. Among synthetic biomaterials Poly-ε- Caprolactone (PCL) is very attractive bioresorbable polyester due to its high permeability, biodegradability and capacity to be blended with other biopolymers. Thanks to its ability to naturally degrade in tissues, PCL has a great potential as a new material for implantable biomedical micro devices. This work focuses on the establishment of a micro fabrication process, by integrating lithography and micromolding fabrication techniques, for the realization of 3D microstructure PCL devices. Scaffold surface exhibits a combination in the patterned length scale; cylindrical pillars of 10 μm height and 10 μm diameter are arranged in a hexagonal lattice with periodicity of 30 μm and their sidewalls are nano-sculptured, with a regular pattern of grooves leading to a spatial modulation in the z direction. In order to demonstrate that these biocompatible pillared PCL substrates are suitable for a proper cell growth, NIH/3T3 mouse embryonic fibroblasts were seeded on them and cells key adhesion parameters were evaluated. Scanning Electron Microscopy and immunofluorescence analysis were carried out to check cell survival, proliferation and adhesion; cells growing on the PCL substrates appeared healthy and formed a well-developed network in close contact with the micro and nano features of the pillared surface. Those 3D scaffolds could be a promising solution for a wide range of applications within tissue engineering and regenerative medicine applications.