alexa Development of a Gaseous Nitrous Acid Generation System for Animal Exposure Experiments
ISSN: 2161-0495

Journal of Clinical Toxicology
Open Access

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Research Article

Development of a Gaseous Nitrous Acid Generation System for Animal Exposure Experiments

Masayuki Ohyama1*, Kenji Oka2, Shuichi Adachi3 and Norimichi Takenaka4

1Department of Environmental Health, Osaka Prefectural Institute of Public Health, Japan

2Department of Research Institute of Environment, Agriculture and Fisheries, Osaka Prefectural Government, Japan

3Department of Public Health, Sagami Women’s University, Japan

4Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Japan

*Corresponding Author:
Ohyama M
Department of Environmental Health
Osaka Prefectural Institute of Public Health
1-3-69, Nakamichi, Higashinari-ku, Osaka 537-0025, Japan
Tel: +81-6-6972-1321
Fax: +81-6-6972-2393
E-mail: [email protected]

Received date: May 28, 2013; Accepted date: June 24, 2013; Published date: June 27, 2013

Citation: Ohyama M, Oka K, Adachi S, Takenaka N (2013) Development of a Gaseous Nitrous Acid Generation System for Animal Exposure Experiments. J Clin Toxicol 3:165. doi: 10.4172/2161-0495.1000165

Copyright: © 2013 Ohyama M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Although a flow-type generation system for obtaining gaseous nitrous acid (HONO) at parts-per-billion levels has already been reported, higher gaseous HONO concentrations on the order of parts per million (ppm) are necessary for use in animal exposure experiments, which are conducted to examine certain biological effects. We have developed a flow-type generation system to produce gaseous HONO at ppm levels for animal exposure experiments. This system is based on spraying a mixture of aqueous sodium nitrite with an acid in a porous polytetrafluoroethylene tube. Herein, we report the technical details and cautionary notes of the manufacturing of the system. The most technical cautionary note pertains the flow-control method in the tube for the gas and water solution.

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