Development of a Highly Sensitive, Fast and Efficient Screening Technique for the Detection of 2,3-Butanediol by Thin Layer ChromatographySaran S1, Yadav S2 and Saxena RK1*
- *Corresponding Author:
- Saxena RK
Technology Based Incubator, University of Delhi South Campus
Biotech Centre, Benito Juarez Road, New Delhi-110021
E-mail: [email protected]
Received date: October 09, 2014; Accepted date: November 24, 2014; Published date: November 30, 2014
Citation: Saran S, Yadav S, Saxena RK (2014) Development of a Highly Sensitive, Fast and Efficient Screening Technique for the Detection of 2,3-Butanediol by Thin Layer Chromatography. J Chromatogr Sep Tech 5:251. doi:10.4172/2157-7064.1000251
Copyright: © 2014 Saran S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The interest in microbial production of 2,3-butanediol is based on the awareness that 2,3 butanediol is a promising bulk chemical due to its extensive industrial applications. Here, we report a novel method for the detection of 2,3-butanediol. The proposed method has a wide applicability to harness the commercial potential of microorganisms which produce 2,3-butanediol as the end product. Experimentally 32 bacterial strains were screened for 2,3-butanediol production. After 72 h the samples were spotted on thin-layer chromatography plates and ran in a solvent system comprising of hexane:ethyl-acetate:glacial acetic acid in the ratio of 70:30:1.5, followed by colour development using vanillin reagent. The appearance of a blue-colored spot of 2,3-butanediol with a retention factor (Rf) of 0.68 corresponds to the Rf value of the standard 2,3-butanediol which forms the basis for the selection of 2,3-butanediol producers. Apart from being a rapid detection system the proposed method is highly sensitive as it was able to detect a concentration as low as 1.0 mg/ml and its authenticity was reconfirmed by GC and GC-MS. Low cost of this method provides an effective support for 2,3-butanediol detection at any scale.