alexa Development of a Method to Detect Viral RNA Sequences From Cultured Cells by Combining Size Fraction and a Rapid Determination System for Viral RNA Sequences (RDV) | Abstract
ISSN: 2157-7579

Journal of Veterinary Science & Technology
Open Access

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Research Article

Development of a Method to Detect Viral RNA Sequences From Cultured Cells by Combining Size Fraction and a Rapid Determination System for Viral RNA Sequences (RDV)

Shumpei Watanabe1, Tetsuya Mizutani2*, Kouji Sakai3, Itoe Iizuka2, Tomoyuki Shiota2, Yusuke Sayama2, Shumpei Tsuda1, Kentaro Kato1,Shuetsu Fukushi2, Masayuki Saijo2, Ichiro Kurane2, Shigeru Morikawa2 and Hiroomi Akashi1

1Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

2Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan

3Division of Experimental Animals Research, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan

*Corresponding Author:
Dr. T. Mizutani
Department of Virology 1
National Institute of Infectious Diseases
Gakuen 4-7-1, Musashimurayama
Tokyo, Japan
Tel: +81- 42-561-0771
Fax: +81-42-565-3315
E-mail: [email protected]

Accepted date: September 28, 2010; Published date: September 30, 2010

Citation: Watanabe S, Mizutani T, Sakai K, Iizuka I, Shiota T, et al. (2010) Development of a Method to Detect Viral RNA Sequences From Cultured Cells by Combining Size Fraction and a Rapid Determination System for Viral RNA Sequences (RDV). J Veterinar Sci Technol 1:103. doi:10.4172/2157-7579.1000103

Copyright: © 2010 Watanabe S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

By combining size-fraction and a rapid determination system for viral RNA sequences (RDV), a method for sequence independent detection from virus-infected cultured cells was developed as “RDV-size fraction (RDV-SF)”. Using RDV- SF, we succeeded in detecting nucleotide fragment sequences of feline calicivirus (FCV) and Yokose virus (YOKV) from Vero E6 cells infected with FCV and YOKV, respectively. RDV-SF was useful in identifying RNA viruses with a genome size of more than 5 kb from virus-infected cultured cells.

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