alexa Development of a New Dermatophyte-Detection Device using Immunochromatography | OMICS International
ISSN: 2168-9784

Journal of Medical Diagnostic Methods
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Development of a New Dermatophyte-Detection Device using Immunochromatography

Hiroaki Wakamoto1* and Masahiro Miyamoto2

1Life Chemicals Launch Office, JNC Corporation, Tokyo, Japan

2Yokohama Research Center, JNC Corporation, Kanagawa, Japan

Corresponding Author:
Hiroaki Wakamoto
Life Chemicals Launch Office
JNC Corporation, Shin-Otemachi Building
2-1, Otemachi 2-chome, Chiyoda-ku, Tokyo 1008105, Japan
Tel: 81332436284
E-mail: [email protected]

Received Date: May 30, 2016; Accepted Date: Jun 20, 2016; Published Date: Jun 27, 2016

Citation: Wakamoto H, Miyamoto M (2016) Development of a New Dermatophyte-Detection Device using Immunochromatography. J Med Diagn Meth 5:216. doi:10.4172/2168-9784.1000216

Copyright: © 2016 Wakamoto H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: The evaluation method for the diagnosis of tinea unguium is mainly microscopy. New antidermatophyte monoclonal antibody have been developed that recognizes the cell wall polysaccharide antigen of filamentous fungi, including dermatophytes, and applied it to immunochromatography. Some scientists focused on this technology, improved it, and determined that this test method provided useful information for a diagnosis of tinea unguium by analyzing large numbers of clinical specimens. However, these studies were carried out by a tentative method.

Objective: Establishment of the usage of this test strip for clinical use and the certification of the stability during long-term storage.

Methods: Various fungi and bacteria were cultured and extracted by extraction buffer to evaluate the reactivity and measurement range of dermatophytes. Trichophyton rubrum was used as a quality control antigen, and detection limits were set at 0.5 μg/ml as weak positive and set at a 100-fold concentration (50 μg/ml) as strong positive. Nail samples, which had already been identified as positive or negative using the test strips, were randomly selected, cut into fine pieces and mixed. Both positive and negative standard nail samples were prepared.

Results: Positive or negative results were obtained from test lines for 5 to 60 minutes at 1 to 30°C. The detection limits of dried 7 samples of dermatophytes were 0.3 to 3 μg/ml. The results of reactivity showed that 8 of dermatophytes were positive. On the other hand, the test strip did not react to Malassezia or Candida species and bacterial strains. Some of Aspergillus, Penicillium, and Fusarium, which are usually not resident microbiota that inhabit nails in healthy persons, showed a positive reaction. The antifungal agents, terbinafine, griseofulvin, and itraconazole did not affect the results. All 3 lots of the test strips met the standards set by the method of quality control after they were kept in storage at 30°C for up to 22 months.

Conclusion: A newly developed dermatophyte-detection device was easy to use, gave rapid results and high reproducibility, and was stable for 22 months at 30°C.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version