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Development of an Amperometric Biosensor Based on Peroxidases from Brassica napus for the Determination of Ochratoxin a Content in Peanut Samples | OMICS International | Abstract
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

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Research Article

Development of an Amperometric Biosensor Based on Peroxidases from Brassica napus for the Determination of Ochratoxin a Content in Peanut Samples

Eduardo A. Ramírez1, Adrián M. Granero2, María A. Zón2* and Héctor Fernández2*

1Laboratorio de Nanoscopías y Fisicoquímica de Superficies, Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Diagonal 113 y calle 64 - La Plata, Argentina

2Departamento de Química, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal No. 3 (5800) - Río Cuarto, Argentina.

*Corresponding Author:
Héctor Fernández
Department of Chemistry
University of Río Cuarto
Tel: 54 358 467 6440
Fax: 54 358 467 6233
E-mail: [email protected] exa.unrc.edu.ar

María A. Zón
Department of Chemistry
University of Río Cuarto
Tel: 54 358 467 6440
Fax: 54 358 467 6233
E-mail: [email protected]

Received Date: July 12, 2011; Accepted Date: August 12, 2011; Published Date: August 15, 2011

Citation: Ramírez EA, Granero AM, Zón MA, Fernández H (2011) Development of an Amperometric Biosensor Based on Peroxidases from Brassica napus for the Determination of Ochratoxin a Content in Peanut Samples. J Biosens Bioelectron S3:001. doi: 10.4172/2155-6210.S3-001

Copyright: © 2011 Ramírez EA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

An amperometric biosensor based on Brassica napus hairy roots peroxidases to determine ochratoxin A is proposed by the first time. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. The method employs a dialysis membrane covered, peroxidases entrapped and ferrocene-embedded carbon paste electrode (PBHR-Fc-CP) and is based on the fact that the decreased amount of H 2 O 2 produced by the action of PBHR is proportional to the oxidised amount of ochratoxin A in the solution. The PBHR- Fc-CP biosensors showed a very good stability during at least five days. The reproducibility and the repeatability were 7.7% and 5.4%, respectively, showing a good biosensor performance. The calibration curve was linear in the ochratoxin A concentration range from 1 x 10 -8 to 1,4 x 10 -4 mol L -1 . The lowest concentration value measured experimentally for a signal to noise ratio of 3:1 was 5.7 x 10-9 mol L -1 .

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