alexa Development of an Improved Porcine Embryo Culture Medium for Cloning, Transgenesis and Embryonic Stem Cell Isolation | OMICS International | Abstract
ISSN: 2168-9849

Cloning & Transgenesis
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Research Article

Development of an Improved Porcine Embryo Culture Medium for Cloning, Transgenesis and Embryonic Stem Cell Isolation

Luke FS Beebe, Stephen M McIlfatrick, Ivan M Vassiliev and Mark B Nottle*
Reproductive Biotechnology Group, Research Centre for Reproductive Health and Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, Faculty of Health Sciences, University of Adelaide, Adelaide, South Australia
Corresponding Author : Mark Nottle
Obstetrics and Gynaecology
Level 6 Medical School North Wing Frome Rd, Adelaide, SA, Australia, 5005
Tel: + 61 8 8303 4087
Fax: + 61 8 8303 4099
E-mail: [email protected]
Received May 14, 2013; Accepted June 28, 2013; Published July 02, 2013
Citation: Beebe LFS, McIlfatrick SM, Vassiliev IM, Nottle MB (2013) Development of an Improved Porcine Embryo Culture Medium for Cloning, Transgenesis and Embryonic Stem Cell Isolation. Clon Transgen 2:107. doi:10.4172/2168-9849.1000107
Copyright: © 2013 Beebe LFS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Work in our laboratory for more than two decades has focussed on the production of genetically modified pigs for xeno transplantion research. More recent work has focussed on the isolation of porcine embryonic stem cells to facilitate this as well as and other research applications. Central to this research has been the production of in vitro Produced (IVP) embryos. These embryos are produced using a twostep culture system based on NCSU23. This culture system which was developed by modifying energy substrate availability and concentrations and by adding non-essential and essential amino acids in a sequential manner. As a result of this work we have developed a culture system that better suits the changing metabolic needs of the pig embryo and produces embryos with relatively high developmental competence compared to the original formulation. These embryos can be used for a range of research applications including the isolation of embryonic stem cells.

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