Development of Celecoxib Complexes: Characterization and Cytotoxicity Studies in MCF-7Md Aftab Alam1, Mohd Aamir Mirza1, Sushama Talegaonkar1, Amulya K Panda2 and Zeenat Iqbal1*
- *Corresponding Author:
- Dr. Zeenat Iqbal
Asst. Professor, Department of Pharmaceutics
Jamia Hamdard, New Delhi, India-110062
E-mail: [email protected]
Received date: January 03, 2013; Accepted date: January 23, 2013; Published date: January 25, 2013
Citation: Alam MA, Mirza MA, Talegaonkar S, Panda AK, Iqbal Z (2013) Development of Celecoxib Complexes: Characterization and Cytotoxicity Studies in MCF-7. Pharmaceut Anal Acta 4:211. doi: 10.4172/2153-2435.1000211
Copyright: © 2013 Alam MA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The objective of the study was to compare the complexing ability of Celecoxib with different complexing agents. Inclusion complex of Celecoxib is well established in literature but its evaluation in terms of thermodynamics was not studied elaborately. Hence after characterization of complexes with Differential scanning calorimeter, X-ray diffraction, NMR and % complexation efficiency, its solvation energetics and thermodynamics were determined. Complexation ability of celecoxib with caffeine was also evaluated, where non-inclusion (charge transfer) mechanism is involved. So, these two mechanisms of complexation were also tested with respect to each other. In this manuscript, new complexing agents (humic acid and fulvic acid) were also introduced for Celecoxib. These lie in the category of natural organic matter and we explored an indigenous source (Shilajit) to extract it. Other agents used for the development of complex were HP-β-CD and β-CD. Release mechanism of drug from complexes was studied by in vitro release studies. MTT assays were also performed to assess in vitro cell toxicity potential of the complexes in comparision to neat drug.