alexa Development of Deuterated-Leucine Labeling with Immunoprecipitation to Analyze Cellular Protein Complex
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Development of Deuterated-Leucine Labeling with Immunoprecipitation to Analyze Cellular Protein Complex

Shufang Liang1,2*, Xuejiao Xu2 , Haojie Lu2 , Pengyuan Yang2

1state Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China

2Department of Chemistry & Institute of Biomedical Science, Fudan University, Shanghai 200433, China

*Corresponding Author:
Dr. Shufang Liang.
State Key Laboratory of Biotherapy
West China Hospital, Sichuan University
1# Keyuan Road 4, Gaopeng Street
Chengdu 610041, China
Fax : +86 28 85164060
E-mail: [email protected]

Received Date: August 02, 2008; Accepted Date: September 04, 2008; Published Date: September 13, 2008

Citation: Shufang L , Xuejiao X , Haojie L , Pengyuan Y (2008) Development of Deuterated-Leucine Labeling with Immunoprecipitation to Analyze Cellular Protein Complex. J Proteomics Bioinform 1:293-301. doi:10.4172/jpb.1000037

Copyright: © 2008 Shufang L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



The deuterated-leucine (Leu-d 3 ) labeling is one kind of stable isotope labeling by amino acids in cell culture (SILAC), which has been widely used to compar e and quantify pr otein r elative expr ession. W e expanded an integrated immunoprecipitation (IP) coupled with SILAC approach (SILAC-IP) to differentiate the specific bind- ing par tners associated with a bait pr otein in two populations of cells. By this SILAC-IP strategy , the identified specific-binding proteins were quantified by tracking pairs of Leu-d 3 labeled and unlabeled peptides from the mass spectra, which could differentiate specific-binding proteins from nonspecific partners in high confidence. W e applied SILAC-IP method to dif fer entiate specific-binding pr oteins associated with 14-3-3â in human hepato- cellular carcinoma cell line QGY7703 between those in the liver cell line QSG7701. The proteins including HSP86, SKB1hs, GADPH and MEP50 were identified to associate with 14-3-3â in QGY7703 with high binding level than in QSG7701 cells.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version