Development of Indigenous Bio-sensing Methodology for Rapid and Low Cost Endotoxin Detection System
- *Corresponding Author:
- Das AP
Centre of Biotechnology, Siksha O Anusandhan University
Khandagiri Square, Bhubaneswar, India
E-mail: [email protected]
Received date: April 01, 2016; Accepted date: April 20, 2016; Published date: April 26, 2016
Citation:Bal B, Armstrong PB, Das AP (2016) Development of Indigenous Biosensing Methodology for Rapid and Low Cost Endotoxin Detection System. Sensor Netw Data Commun S1:005. doi: 10.4172/2090-4886.S1-005
Copyright: © 2016 Bal B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Endotoxin is a signature molecule of gram-negative bacteria and is clinically significant as the agent of gramnegative sepsis, a disease condition with high mortality. This study describes an enzyme-substrate reaction using the distilled water lysate of the granular hemocytes (amebocytes) of the mangrove horseshoe crab (Tachypleus gigas), a native of the Bay of Bengal, and a chromogenic peptide which results in the production of yellow spectroscopically monitored product in the presence of endotoxin. The assay is complete within 30 min and shows a lower detection limit of 0.2 EU/mL. The novelty of our approach is the use of mangrove horseshoe crabs are the source of amebocyte lysate instead of the American horseshoe crab (Limuluspoly phemus).The anticipated methodology offers advantages for the South Asian market that include a low cost due to use of indigenous reagents. This method shows exquisite sensitivity and provides a rapid assessment of LPS concentrations. We assume that the commercial development of our assay will reduce the cost of a single chromogenic endotoxin detection test as compared to the cost of present imported kits presently available in Asian and Indian market.