alexa Development of Prevascularized Cell Sheets Using Single Bone Marrow Mesenchymal Stem Cell Source for Tissue Regeneration
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Development of Prevascularized Cell Sheets Using Single Bone Marrow Mesenchymal Stem Cell Source for Tissue Regeneration

Qin Li1, Yuxia Feng2, Dan Zhang1, Jin Ren1, Dongyang Ma2* and Liling Ren1*

1School of Stomatology, Lanzhou University, China

2Lanzhou General Hospitals, Lanzhou Command of PLA, China

*Corresponding Authors:
Liling Ren
School of Stomatology, Lanzhou University, China
E-mail: [email protected]

Dongyang Ma
Lanzhou General Hospital
Lanzhou Command of PLA, Gansu 730000, China
E-mail: [email protected]

Received Date: June 30, 2016; Accepted Date: July 20, 2016; Published Date: July 27, 2016

Citation: Li Q, Feng Y, Zhang D, Ren J, Ma D, et al. (2016) Development of Prevascularized Cell Sheets Using Single Bone Marrow Mesenchymal Stem Cell Source for Tissue Regeneration. J Stem Cell Res Ther 6:351. doi: 10.4172/2157-7633.1000351

Copyright: © 2016 Li Q, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Developing a vascular network for tissue engineering constructs is still a challenge. Cell-sheet technology has already brought promising potential in vascularization. However, endothelial cell source for vascularization is limited due to the difficulties in obtaining endothelial cells from peripheral blood or bone marrow. In this study, we differentiated rabbit bone marrow mesenchymal stem cells (rBMSCs) into endothelial like cells (ECs) by adding vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF) into the culture medium of BMSCs. Then, the induced ECs were seeded on rBMSCs sheets to form vascularized ECs/BMSCs cell sheets. Results show that ECs on the undifferentiated BMSCs sheets formed networks in vitro and in vivo. In vivo results demonstrated that the quantity of vessels in the non-prevascularized constructs was lower than that of prevascularized constructs. Furthermore, the prevascularized rBMSCs sheet constructs induced functional anastomosis with the host vasculature. These results suggested that using BMSCs as an endothelial cell source and cell sheet technology to create prevascularized cell sheet brings a promising potential for tissue regeneration.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords