alexa Development of Prevascularized Cell Sheets Using Single Bone Marrow Mesenchymal Stem Cell Source for Tissue Regeneration
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
Open Access

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Research Article

Development of Prevascularized Cell Sheets Using Single Bone Marrow Mesenchymal Stem Cell Source for Tissue Regeneration

Qin Li1, Yuxia Feng2, Dan Zhang1, Jin Ren1, Dongyang Ma2* and Liling Ren1*

1School of Stomatology, Lanzhou University, China

2Lanzhou General Hospitals, Lanzhou Command of PLA, China

*Corresponding Authors:
Liling Ren
School of Stomatology, Lanzhou University, China
E-mail: [email protected]

Dongyang Ma
Lanzhou General Hospital
Lanzhou Command of PLA, Gansu 730000, China
E-mail: [email protected]

Received Date: June 30, 2016; Accepted Date: July 20, 2016; Published Date: July 27, 2016

Citation: Li Q, Feng Y, Zhang D, Ren J, Ma D, et al. (2016) Development of Prevascularized Cell Sheets Using Single Bone Marrow Mesenchymal Stem Cell Source for Tissue Regeneration. J Stem Cell Res Ther 6:351. doi: 10.4172/2157-7633.1000351

Copyright: © 2016 Li Q, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Developing a vascular network for tissue engineering constructs is still a challenge. Cell-sheet technology has already brought promising potential in vascularization. However, endothelial cell source for vascularization is limited due to the difficulties in obtaining endothelial cells from peripheral blood or bone marrow. In this study, we differentiated rabbit bone marrow mesenchymal stem cells (rBMSCs) into endothelial like cells (ECs) by adding vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF) into the culture medium of BMSCs. Then, the induced ECs were seeded on rBMSCs sheets to form vascularized ECs/BMSCs cell sheets. Results show that ECs on the undifferentiated BMSCs sheets formed networks in vitro and in vivo. In vivo results demonstrated that the quantity of vessels in the non-prevascularized constructs was lower than that of prevascularized constructs. Furthermore, the prevascularized rBMSCs sheet constructs induced functional anastomosis with the host vasculature. These results suggested that using BMSCs as an endothelial cell source and cell sheet technology to create prevascularized cell sheet brings a promising potential for tissue regeneration.


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