DEVELOPMENT OF PROTOCOL FOR MICRO PROPAGATION OF GYNOECIOUS BITTER GOURD (MOMORDICA CHARANTIA L).
|A.K.Verma*, Manish Kumar, Shubhankar Tarafdar, Rajani Singh and S.Thakur
Aditya Biotech Lab & Research Pvt. Ltd. Raipur, Chhattisgarh. Pin 492001 India
|Corresponding Author: A.K.Verma, Tel: +91 8871036809, E-mail: [email protected]|
|Received: 14 August 2014 Accepted: 14 October 2014|
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Bitter gourd (Momordica charantia.L) is an important cucurbitaceous crop. The occurrence of gynaecium in bitter gourd is very rare and generally they have monoecious sex form. Recent identification of some gynoecious lines showed their advantages in hybrid breeding programme. Our present investigation outlines the in vitro propagation of monoecious line of bitter gourd. The auxiliary bud and apical bud of identified gynoecious lines of bitter gourd were cultured on MS medium for initiation. After 3rd subculture stage the average number of shoots and shoot length of bitter gourd was recorded 3.4 and 2.7 cm in auxiliary bud (AXBG) and 2.8 and 2.1 cm in apical bud bitter gourd (APBG) explants respectively. Maximum shoot length was obtained in ABG-6 medium. (½ MS supplemented with 0.5 mg/ml BAP), during the third subculture stage. Rooting was best observed in RBG- 7 medium (½ MS supplemented with 1.0mg/ml IBA). When transferred to the primary hardening stage 44% of the plants were survived successfully. For secondary hardening, primary hardened plants were transferred to polybags containing soil, FYM and sand (3:2:1), 50 % of the plants were survived.